Document Detail


Acquired potential N-glycosylation sites within the tumor-specific immunoglobulin heavy chains of B-cell malignancies.
MedLine Citation:
PMID:  15136216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVES: Among B-cell malignancies, follicular lymphomas (FL) more frequently show acquired, potential N-glycosylation sites (AGS) within tumor-specific immunoglobulin. The aim of this study was to extend this observation and to evaluate the pattern of presentation of AGS within five different forms of B-cell lymphoma. DESIGN AND METHODS: We sequenced the tumor-specific immunoglobulin heavy chain variable region fragment, including complementarity-determining regions 2 and 3, of forty-seven consecutive patients with a B-cell malignancy enrolled in idiotype vaccine clinical trials. This sequencing approach is known to allow the identification of most AGS. We then statistically analyzed differences in presentation pattern, in terms of tumor histology, immunoglobulin isotype, AGS location and amino acid composition. RESULTS: All twenty-four FL cases presented with at least one AGS, whereas the vast majority of four B-cell lymphoma types other than FL did not. The non- FL group of tumors included four cases of Burkitt's lymphoma, six of diffuse large cell lymphoma, seven mantle cell lymphomas and six small lymphocytic lymphomas. Most IgM-bearing follicular lymphoma cases featured their AGS within complementarity-determining region 2, as opposed to those bearing an IgG, which mostly displayed the AGS within complementarity-determining region 3. The vast majority of AGS located within either complementarity-determining region ended with a serine residue, whereas those located within framework regions mostly featured threonine as the last amino acid residue. INTERPRETATION AND CONCLUSIONS: In our series, all cases of FL had AGS within their tumor-specific immunoglobulin heavy chain variable regions. In contrast, most B-cell malignancies other than FL did not. Further studies are warranted in order to establish the possible meaning of these findings in terms of disease pathogenesis, their diagnostic value in doubtful cases and their potential implications for immunotherapy.
Authors:
Natalia Zabalegui; Ascensión López-Díaz de Cerio; Susana Inogés; Mercedes Rodríguez-Calvillo; Javier Pérez-Calvo; Milagros Hernández; Jesús García-Foncillas; Salvador Martín-Algarra; Eduardo Rocha; Maurizio Bendandi
Related Documents :
8415586 - Brain lymphomas of immunocompetent and immunocompromised patients: study of the associa...
616316 - Decreased amniotic fluid peroxidase in malignancy of the cervix.
7653176 - Relation of p53 tumor suppressor protein expression to human papillomavirus (hpv) dna a...
17915676 - Prognostic significance of tumor-infiltrating lymphocytes in oropharyngeal cancer.
25006296 - Metastatic perivascular epithelioid cell tumor responding to mammalian target of rapamy...
20356686 - Melanotic schwannoma: a case with strong cd34 expression, with histogenetic implications.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Haematologica     Volume:  89     ISSN:  1592-8721     ISO Abbreviation:  Haematologica     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-11     Completed Date:  2006-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417435     Medline TA:  Haematologica     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  541-6     Citation Subset:  IM    
Affiliation:
Cell Therapy Area, Department of Hematology, University Clinic, University of Navarre, Avda. Pio XII, 36 - 31008 Pamplona, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Glycosylation*
Humans
Immunoglobulin Heavy Chains / analysis*,  genetics
Lymphoma, B-Cell / chemistry*
Lymphoma, Follicular / chemistry
Lymphoma, Mantle-Cell / chemistry
Molecular Sequence Data
Mutation
Neoplasm Proteins / analysis*,  genetics
Retrospective Studies
Chemical
Reg. No./Substance:
0/Immunoglobulin Heavy Chains; 0/Neoplasm Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Prognostic impact of early response to induction therapy as assessed by multiparameter flow cytometr...
Next Document:  Patients with CD45 negative multiple myeloma receiving high-dose therapy have a shorter survival tha...