| Acoustically detectable cellular-level lung injury induced by fluid mechanical stresses in microfluidic airway systems. | |
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MedLine Citation:
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PMID: 18006663 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a microfabricated airway system integrated with computerized air-liquid two-phase microfluidics that enables on-chip engineering of human airway epithelia and precise reproduction of physiologic or pathologic liquid plug flows found in the respiratory system. Using this device, we demonstrate cellular-level lung injury under flow conditions that cause symptoms characteristic of a wide range of pulmonary diseases. Specifically, propagation and rupture of liquid plugs that simulate surfactant-deficient reopening of closed airways lead to significant injury of small airway epithelial cells by generating deleterious fluid mechanical stresses. We also show that the explosive pressure waves produced by plug rupture enable detection of the mechanical cellular injury as crackling sounds. |
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Authors:
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Dongeun Huh; Hideki Fujioka; Yi-Chung Tung; Nobuyuki Futai; Robert Paine; James B Grotberg; Shuichi Takayama |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-11-15 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 104 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-29 Completed Date: 2008-01-15 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 18886-91 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering and Macromolecular Science, University of Michigan, Ann Arbor, MI 48109-2099, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acoustics
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instrumentation Air Basement Membrane / physiology Cell Differentiation Cell Division Cell Survival Cells, Cultured / physiology Epithelial Cells / physiology Epithelium / injuries*, physiopathology Equipment Design Humans Lung Diseases / etiology*, physiopathology Microfluidic Analytical Techniques Microfluidics* Perfusion Pulmonary Surfactants Shear Strength Stress, Mechanical* Tissue Engineering / instrumentation |
| Grant Support | |
ID/Acronym/Agency:
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HL084370-01/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Pulmonary Surfactants |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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