Document Detail

Acoustic cell filter for high density perfusion culture of hybridoma cells.
MedLine Citation:
PMID:  7764489     Owner:  NLM     Status:  MEDLINE    
We have developed a flow-through device which uses high frequency, low energy ultrasonic resonance fields to transiently aggregate hybridoma cells and return them by sedimentation to a perfusion bioreactor. The system retained up to 99 percent of the inflowing viable cells with no measurable effect on viability. Viable cells were selectively retained at up to 3 percent higher efficiency than nonviable cells. A stirred tank bioreactor was operated for 700 hours with acoustic cell recycle. Concentrations greater than 5 x 10(7) cells/ml were attained with a 5-fold increase in antibody concentration and a 70-fold increase in volumetric productivity compared with batch culture.
F Trampler; S A Sonderhoff; P W Pui; D G Kilburn; J M Piret
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bio/technology (Nature Publishing Company)     Volume:  12     ISSN:  0733-222X     ISO Abbreviation:  Biotechnology (N.Y.)     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-04-07     Completed Date:  1994-04-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8309273     Medline TA:  Biotechnology (N Y)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  281-4     Citation Subset:  B    
Sonofloc Biotech Inc., Richmond, BC, Canada.
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MeSH Terms
Cell Aggregation
Cell Count
Cell Separation / methods*
Cell Survival
Cells, Cultured
Culture Media
Filtration / instrumentation*
Hybridomas / cytology*
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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