| Aclarubicin-induced apoptosis and necrosis in cells derived from human solid tumours. | |
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MedLine Citation:
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PMID: 20399885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, we investigated the response of A549 (non-small cell lung-cancer), HepG2 (human hepatoma) and MCF-7 (human breast adenocarcinoma) cell lines to treatment with aclarubicin (ACL). The aim of this research was to compare the ability of ACL to induce apoptosis or necrosis in solid tumours. The mode of cell death induced by ACL was evaluated by flow-cytometry and fluorescence microscopy. We show that the drug induced both apoptosis and necrosis in the cells. Apoptotic cell death was associated with morphological changes, DNA fragmentation, changes in activity of poly(ADP-ribose)polymerase (PARP) and drug-mediated activation of caspase-3 and caspase-8. The occurrence of all these events was time-dependent. The extent of apoptosis was also dependent on the kind of cell line, the sensitivity to ACL and the intracellular drug content. This study demonstrates that the cells most sensitive to ACL, A549, accumulated a significantly higher level of the drug and were also more susceptible to apoptosis than the other cells. In contrast, the relatively less sensitive HepG2 and MCF-7 cell lines appeared more resistant to apoptosis induction. On the basis of these results, it seems that aclarubicin is able to induce apoptosis in human solid tumours. |
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Authors:
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Aneta Rogalska; Marzena Szwed; Zofia Jóźwiak |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-24 |
Journal Detail:
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Title: Mutation research Volume: 700 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-15 Completed Date: 2010-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-10 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Thermobiology, University of Lodz, Banacha 12/16 st., 90-237 Lodz, Poland. zychan@biol.uni.lodz.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aclarubicin
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metabolism,
pharmacology* Antibiotics, Antineoplastic / pharmacology* Apoptosis / drug effects* Caspase 3 / metabolism Caspase 8 / metabolism DNA Fragmentation / drug effects Dose-Response Relationship, Drug Drug Screening Assays, Antitumor Humans In Situ Nick-End Labeling Necrosis / chemically induced* Poly(ADP-ribose) Polymerases Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 57576-44-0/Aclarubicin; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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