| Acinetobacter strains IH9 and OCI1, two rhizospheric phosphate solubilizing isolates able to promote plant growth, constitute a new genomovar of Acinetobacter calcoaceticus. | |
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MedLine Citation:
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PMID: 19467815 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During a screening of phosphate solubilizing bacteria (PSB) in agricultural soils, two strains, IH9 and OCI1, were isolated from the rhizosphere of grasses in Spain, and they showed a high ability to solubilize phosphate in vitro. Inoculation experiments in chickpea and barley were conducted with both strains and the results demonstrated their ability to promote plant growth. The 16S rRNA gene sequences of these strains were nearly identical to each other and to those of Acinetobacter calcoaceticus DSM 30006(T), as well as the strain CIP 70.29 representing genomospecies 3. Their phenotypic characteristics also coincided with those of strains forming the A. calcoaceticus-baumannii complex. They differed from A. calcoaceticus in the utilization of l-tartrate as a carbon source and from genomospecies 3 in the use of d-asparagine as a carbon source. The 16S-23S intergenic spacer (ITS) sequences of the two isolates showed nearly 98% identities to those of A. calcoaceticus, confirming that they belong to this phylogenetic group. However, the isolates appeared as a separate branch from the A. calcoaceticus sequences, indicating their molecular separation from other A. calcoaceticus strains. The analysis of three housekeeping genes, recA, rpoD and gyrB, confirmed that IH9 and OCI1 form a distinct lineage within A. calcoaceticus. These results were congruent with those from DNA-DNA hybridization, indicating that strains IH9 and OCI1 constitute a new genomovar for which we propose the name A. calcoaceticus genomovar rhizosphaerae. |
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Authors:
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Alvaro Peix; Elke Lang; Susanne Verbarg; Cathrin Spröer; Raúl Rivas; Ignacio Santa-Regina; Pedro F Mateos; Eustoquio Martínez-Molina; Claudino Rodríguez-Barrueco; Encarna Velázquez |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-20 |
Journal Detail:
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Title: Systematic and applied microbiology Volume: 32 ISSN: 1618-0984 ISO Abbreviation: Syst. Appl. Microbiol. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-20 Completed Date: 2009-09-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306133 Medline TA: Syst Appl Microbiol Country: Germany |
Other Details:
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Languages: eng Pagination: 334-41 Citation Subset: IM |
Affiliation:
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Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/FJ694758; FJ694759; FJ694760 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acinetobacter calcoaceticus
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classification*,
isolation & purification*,
physiology Asparagine / metabolism Bacterial Typing Techniques Cluster Analysis DNA Gyrase / genetics DNA, Bacterial / chemistry, genetics DNA, Ribosomal / chemistry, genetics DNA, Ribosomal Spacer / chemistry, genetics DNA-Directed RNA Polymerases / genetics Molecular Sequence Data Nucleic Acid Hybridization Phosphates / metabolism* Phylogeny Plant Roots / microbiology* Poaceae / growth & development*, microbiology* RNA, Ribosomal, 16S / genetics Rec A Recombinases / genetics Sequence Analysis, DNA Sigma Factor / genetics Soil Microbiology* Spain Tartrates / metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Ribosomal; 0/DNA, Ribosomal Spacer; 0/Phosphates; 0/RNA, Ribosomal, 16S; 0/Sigma Factor; 0/Tartrates; 526-83-0/tartaric acid; 7006-34-0/Asparagine; EC 2.7.7.-/RNA polymerase sigma 70; EC 2.7.7.-/Rec A Recombinases; EC 2.7.7.6/DNA-Directed RNA Polymerases; EC 5.99.1.-/DNA Gyrase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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