| Acinar cells are target cells for androgens in mouse submandibular glands. | |
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MedLine Citation:
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PMID: 9562575 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The variable coding sequence (VCS) multigene family encodes diverse salivary proteins, such as the SMR1 prohormone and the PR-VB1 proline-rich protein in the rat. In situ hybridization was used to study the cell-specific expression of two new mouse VCS genes, Vcs1 and Vcs2. We show that the Vcs1 transcripts, which code for a proline-rich protein, MSG1, are highly abundant in male and female parotid glands, in which they are specifically detected in acinar cells. No expression was seen in the submandibular or sublingual glands. In contrast, Vcs2 transcripts were found only in the acinar cells of the submandibular glands (SMGs) of male mice, in which they are expressed in response to androgens. Expression was found to be heterogeneous within acinar structures. No Vcs2 transcripts were detected in the SMGs of females or castrated males by Northern blot, RNase protection, or in situ hybridization. Androgen administration to females or castrated males induced expression at a level comparable to that of intact males. The Vcs2 gene is the first example of a mouse androgen-regulated gene that is expressed in SMG acinar cells. This result, in addition to our previous observation on SMR1 expression in rats, demonstrates that both acinar cells and granular convoluted tubule (GCT) cells are target cells for androgen action in rodent SMG. |
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Authors:
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M Señorale-Pose; A Jacqueson; F Rougeon; I Rosinski-Chupin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 46 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 1998 May |
Date Detail:
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Created Date: 1998-05-28 Completed Date: 1998-05-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 669-78 Citation Subset: IM |
Affiliation:
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Unité de Génétique et Biochimie du Développement, URA CNRS 1960, Institut Pasteur, Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Androgens
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physiology* Animals Castration Female Gene Expression Regulation In Situ Hybridization Male Mice Mice, Inbred BALB C Multigene Family* Protein Precursors / genetics RNA, Messenger / metabolism Rats Salivary Proteins and Peptides / genetics* Sex Characteristics Submandibular Gland / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Androgens; 0/Protein Precursors; 0/RNA, Messenger; 0/Salivary Proteins and Peptides; 0/Smr1 protein, mouse; 0/Smr2 protein, mouse; 0/Vcsa1 protein, rat |
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