Document Detail

Acidic substitution of the activation loop tyrosines in TrkA supports nerve growth factor-dependent, but not nerve growth factor-independent, differentiation and cell cycle arrest in the human neuroblastoma cell line, SY5Y.
MedLine Citation:
PMID:  14647472     Owner:  NLM     Status:  MEDLINE    
TrkA is the receptor tyrosine kinase (RTK) for nerve growth factor (NGF) and stimulates NGF-dependent cell survival and differentiation in primary neurons and also differentiation of neuroblastomas and apoptosis of medulloblastomas. We have previously shown that aspartic acid and glutamic acid substitution (AspGlu and GluAsp) of the activation loop tyrosines in TrkA (Tyr(683) and Tyr(684)) supports NGF-independent neuritogenesis and cell survival in PC12 cell-derived nnr5 cells. In this study, the AspGlu and GluAsp mutant Trks have been analysed for their ability to support NGF-independent and NGF-dependent neuritogenesis, proliferation and cell signalling in the human neuroblastoma cell line, SY5Y. We find that the AspGlu and GluAsp mutant Trks support NGF-dependent, but not NGF-independent, autophosphorylation, neuritogenic responses and/or inhibit cell cycle progression. The NGF-dependent neuritogenic responses are lower for the mutant Trks (approximately 30-60% for AspGlu and 50-60% for GluAsp), relative to wild-type TrkA. While both the AspGlu and GluAsp mutant Trks support NGF-dependent transient phosphorylation of Shc, PLCgamma-1, AKT, FRS2, SH2B as well as prolonged MAP kinase activation, the GluAsp mutant induces stronger NGF-dependent tyrosine phosphorylation of FRS2 and SH2B, as well as a stronger reduction in bromodeoxyuridine (BrdU) incorporation. Collectively, these data suggest that neither absolute levels of receptor autophosphorylation, high levels of TrkA expression nor the activation of a specific signalling pathway is dominant and absolutely essential for neuritogenesis and cell cycle arrest of SY5Y cells.
Ela A Gryz; Susan O Meakin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  22     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-12-03     Completed Date:  2003-12-18     Revised Date:  2012-06-22    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  8774-85     Citation Subset:  IM    
Laboratory of Neural Signalling, Cell Biology Group, The Robarts Research Institute, 100 Perth Drive, London, Ontario, Canada N6A 5K8.
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MeSH Terms
Adaptor Proteins, Signal Transducing*
Adaptor Proteins, Vesicular Transport / metabolism
Amino Acid Substitution
Carrier Proteins / chemistry*,  metabolism,  physiology
Cell Cycle
Cell Differentiation
Cell Line, Tumor
Membrane Proteins / chemistry*,  metabolism,  physiology
Mitogen-Activated Protein Kinases / metabolism
Nerve Growth Factor / physiology*
Neurites / physiology
Neuroblastoma / metabolism,  pathology
Phospholipase C gamma
Phosphoproteins / metabolism
Protein-Serine-Threonine Kinases*
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins c-akt
Receptor, trkA*
Shc Signaling Adaptor Proteins
Signal Transduction
Type C Phospholipases / metabolism
Tyrosine / metabolism*
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Adaptor Proteins, Vesicular Transport; 0/Carrier Proteins; 0/FRS2 protein, human; 0/Membrane Proteins; 0/Phosphoproteins; 0/Proto-Oncogene Proteins; 0/SH2B1 protein, human; 0/SHC1 protein, human; 0/Shc Signaling Adaptor Proteins; 55520-40-6/Tyrosine; 9061-61-4/Nerve Growth Factor; EC, trkA; EC protein, human; EC Kinases; EC Proteins c-akt; EC Protein Kinases; EC 3.1.4.-/Type C Phospholipases; EC C gamma

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