| Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength. | |
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MedLine Citation:
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PMID: 291918 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We provide evidence that nucleosomes can assemble in vitro at physiological ionic strength (0.1-0.2 M NaCl/10 mM Tris . HCl, pH 8.0) in the absence of "assembly factors" and that poly(glutamic acid) greatly facilitates chromatin assembly under these conditions. We also show that in the presence of either poly(glutamic acid) or poly(aspartic acid), core histones assemble into octamers at physiological ionic strength. We suggest that it is a property of histones to assemble into octamers upon their interaction with macromolecules containing regions of high negative charge density, and we discuss several implications of this property. |
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Authors:
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A Stein; J P Whitlock; M Bina |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 76 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1979 Oct |
Date Detail:
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Created Date: 1980-01-28 Completed Date: 1980-01-28 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5000-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chickens Chromatin / ultrastructure* Erythrocytes / analysis Histones* / blood Macromolecular Substances Microscopy, Electron Molecular Weight Osmolar Concentration Peptides* |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Histones; 0/Macromolecular Substances; 0/Peptides |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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