Document Detail


Acidic polyanion poly(acrylic acid) prevents calcium oxalate crystal deposition.
MedLine Citation:
PMID:  18563053     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acidic macromolecules inhibit calcium oxalate nucleation, growth, aggregation and attachment to cells in vitro. To test for such an effect in vivo we used osmotic minipumps to continuously infuse several doses of the 5.1 kDa poly(acrylic acid) (pAA(5.1)) into rats fed a diet which causes renal calcium oxalate crystal deposition. Although kidneys of rats receiving the saline control contained calcium oxalate crystals, measured by polarized light microscopy, those of animals given pAA(5.1) had significantly lower numbers of crystals in various zones of the kidney. Delivery of pAA(5.1) to urine was confirmed by measuring excretion of infused biotinylated pAA(5.1). Both the derivatized and unlabelled pAA(5.1) had the same effects on crystallization in vitro. Our study shows that acidic polymers hold promise as effective therapies for kidney stones likely through prevention of calcium oxalate crystal aggregate formation.
Authors:
Jack G Kleinman; Laura J Alatalo; Ann M Beshensky; Jeffrey A Wesson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-06-18
Journal Detail:
Title:  Kidney international     Volume:  74     ISSN:  1523-1755     ISO Abbreviation:  Kidney Int.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-16     Completed Date:  2008-11-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  919-24     Citation Subset:  IM    
Affiliation:
Division of Nephrology, Department of Medicine, Medical College of Wisconsin and the Department of Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295, USA. Kleinman@mcw.edu
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins / administration & dosage,  pharmacokinetics,  pharmacology*
Animals
Calcium Oxalate / metabolism*
Crystallization
Kidney Calculi / drug therapy,  prevention & control*
Male
Polymers / administration & dosage,  pharmacokinetics,  pharmacology*
Rats
Rats, Sprague-Dawley
Urine / chemistry
Grant Support
ID/Acronym/Agency:
DK74741/DK/NIDDK NIH HHS; R01 DK074741-02/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Polymers; 0/polyanions; 25454-23-3/Calcium Oxalate; 9003-01-4/carbopol 940
Comments/Corrections

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