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Acidic lipase Lip I.3 from a Pseudomonas fluorescens-like strain displays unusual properties and shows activity on secondary alcohols.
MedLine Citation:
PMID:  23190193     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIMS: Identification, cloning, expression and characterization of a novel lipase -Lip I.3- from strain Pseudomonas CR-611. METHODS AND RESULTS: The corresponding gene was identified and isolated by PCR-amplification, cloned and expressed in E. coli, and purified by refolding from inclusion bodies. Analysis of the deduced amino acid sequence revealed high homology with members of bacterial lipase family I.3, showing 97% identity to a putative lipase from P. fluorescens Pf0-1, and 93% identity to a crystallized extracellular lipase from Pseudomonas sp. MIS38. A typical C-terminal type I secretion signal and several putative Ca(2+) binding sites were also identified. Experimental data confirmed that Lip I.3 requires Ca(2+) ions for correct folding and activity. The enzyme differs from previously reported family I.3 lipases in optimal pH, being the first acidophilic lipase reported in this family. Furthermore, Lip I.3 shows a strong preference for medium chain fatty acid esters and does not display interfacial activation. When tested for activity on secondary alcohol hydrolysis, LipI.3 displayed higher efficiency on aromatic alcohols rather than on alkyl alcohols. CONCLUSIONS: New family I.3 lipase with unusual properties isolated, cloned and described. This will contribute to a better knowledge of family I.3 lipases, a family that has been scarcely explored, and that might provide a novel source of biocatalysts. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Authors:
Paola Panizza; Nikolia Syfantou; F I Javier Pastor; Sonia Rodríguez; Pilar Díaz
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-29
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
Department of Microbiology, Faculty of Biology, University of Barcelona, Av. Diagonal 645, 08028, Barcelona, Spain; Bioscience Department, Faculty of Chemistry, Universidad de la República, Uruguay.
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