| Acidic buffer or plus cyclosporine a postconditioning protects isolated rat hearts against ischemia-reperfusion injury. | |
| | |
MedLine Citation:
|
PMID: 21310770 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: It is well documented that transient acidosis during reperfusion is protective. The aim of this study was to evaluate the cardioprotection of acidic buffer or plus cyclosporine A in isolated rat hearts after cardioplegic arrest. METHODS: Langendorff-perfused Sprague-Dawley rat hearts were perfused for 20 min with Krebs-Henseleit (K-H) buffer followed by 30 min of crystalloid cardioplegia and 60 min of reperfusion. Control hearts were perfused with Krebs-Henseleit buffer. Acidic buffer post-conditioning hearts were perfused with acidic K-H buffer (pH 6.8) for the first 3 min of reperfusion. Acidic buffer plus cyclosporine A hearts were perfused with K-H acidic buffer (pH 6.8) containing cyclosporine A (0.2 μmol/L) for the first 3 min of reperfusion. RESULTS: Compared with the control group, acidic buffer or plus cyclosporine A post-conditioning significantly improved myocardial performance, decreased cytochrome C release into the cytosol, increased Bcl-2 expression and decreased Bax expression, decreased sensitivity of mPTP-opening to [Ca2+] and the rate of apoptosis after reperfusion. CONCLUSION: These findings suggested that acidic buffer or plus cyclosporine A post-conditioning prevented apoptosis-related mitochondrial permeabilization and provided the myocardial protection after cardioplegic arrest. |
| | |
Authors:
|
Xin Duan; Bingyang Ji; Kun Yu; Jinping Liu; Feilong Hei; Cun Long |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-2-10 |
Journal Detail:
|
Title: Perfusion Volume: - ISSN: 1477-111X ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-2-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8700166 Medline TA: Perfusion Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Fuwai Hospital and Cardiovascular Institute, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, Peoples Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Emerging diagnostic and therapeutic molecular imaging applications in vascular disease.
Next Document: Transitions in Marital Status and Functional Health and Patterns of Intergenerational Coresidence Am...