| Acidic buffer or plus cyclosporine A post-conditioning protects isolated rat hearts against ischemia-reperfusion injury. | |
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MedLine Citation:
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PMID: 21490080 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: It is well documented that transient acidosis during reperfusion is protective. The aim of this study was to evaluate the cardioprotection of acidic buffer or plus cyclosporine A in isolated rat hearts after cardioplegic arrest. METHODS: Langendorff-perfused Sprague-Dawley rat hearts were perfused for 20 min with Krebs-Henseleit (K-H) buffer followed by 30 min of crystalloid cardioplegia and 60 min of reperfusion. Control hearts were perfused with Krebs-Henseleit buffer. Acidic buffer post-conditioning hearts were perfused with acidic K-H buffer (pH 6.8) for the first 3 min of reperfusion. Acidic buffer plus cyclosporine A hearts were perfused with K-H acidic buffer (pH 6.8) containing cyclosporine A (0.2 μmol/L) for the first 3 min of reperfusion. RESULTS: Compared with the control group, acidic buffer or plus cyclosporine A post-conditioning significantly improved myocardial performance, decreased cytochrome C release into the cytosol, increased Bcl-2 expression and decreased Bax expression, decreased sensitivity of mPTP-opening to [Ca2+] and the rate of apoptosis after reperfusion. CONCLUSION: These findings suggested that acidic buffer or plus cyclosporine A post-conditioning prevented apoptosis-related mitochondrial permeabilization and provided the myocardial protection after cardioplegic arrest. |
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Authors:
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Xin Duan; Bingyang Ji; Kun Yu; Feilong Hei; Jinping Liu; Cun Long |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Perfusion Volume: 26 ISSN: 1477-111X ISO Abbreviation: Perfusion Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8700166 Medline TA: Perfusion Country: England |
Other Details:
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Languages: eng Pagination: 245-52 Citation Subset: IM |
Affiliation:
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Fuwai Hospital and Cardiovascular Institute, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, Peoples Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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