Document Detail


Acid-sensing ion channels in acidosis-induced injury of human brain neurons.
MedLine Citation:
PMID:  20216553     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acidosis is a common feature of the human brain during ischemic stroke and is known to cause neuronal injury. However, the mechanism underlying acidosis-mediated injury of the human brain remains elusive. We show that a decrease in the extracellular pH evoked inward currents characteristic of acid-sensing ion channels (ASICs) and increased intracellular Ca(2+) in cultured human cortical neurons. Acid-sensing ion channels in human cortical neurons show electrophysiological and pharmacological properties distinct from those in neurons of the rodent brain. Reverse transcriptase-PCR and western blot detected a high level of the ASIC1a subunit with little or no expression of other ASIC subunits. Treatment of human cortical neurons with acidic solution induced substantial cell injury, which was attenuated by the ASIC1a blockade. Thus, functional homomeric ASIC1a channels are predominantly expressed in neurons from the human brain. Activation of these channels has an important role in acidosis-mediated injury of human brain neurons.
Authors:
Minghua Li; Koichi Inoue; Deborah Branigan; Eric Kratzer; Jillian C Hansen; Jeff W Chen; Roger P Simon; Zhi-Gang Xiong
Related Documents :
22194263 - Bapti and baptism birthdating of neurons in zebrafish.
11335063 - Unbiased stereological quantification of neurons in the human spiral ganglion.
4887743 - Surgery in the rat during electrical analgesia induced by focal brain stimulation.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-03-10
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  30     ISSN:  1559-7016     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-01     Completed Date:  2010-06-17     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1247-60     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acid Sensing Ion Channels
Acidosis / metabolism*
Adult
Aged
Animals
Cerebral Cortex / metabolism*
Female
Gene Expression Regulation*
Humans
Male
Middle Aged
Nerve Tissue Proteins / biosynthesis*
Neurons / metabolism*
Rodentia / metabolism
Sodium Channels / biosynthesis*
Species Specificity
Grant Support
ID/Acronym/Agency:
R01 NS047506/NS/NINDS NIH HHS; R01 NS047506-05A1/NS/NINDS NIH HHS; R01NS47506/NS/NINDS NIH HHS; R01NS50610/NS/NINDS NIH HHS; R56 NS047506/NS/NINDS NIH HHS; R56 NS047506-05A1/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/ASIC1 protein, human; 0/Acid Sensing Ion Channels; 0/Nerve Tissue Proteins; 0/Sodium Channels
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Tridermal tumorigenesis of induced pluripotent stem cells transplanted in ischemic brain.
Next Document:  MicroRNA control of signal transduction.