Document Detail

Acid protease production by solid-state fermentation using Aspergillus oryzae MTCC 5341: optimization of process parameters.
MedLine Citation:
PMID:  19937364     Owner:  NLM     Status:  MEDLINE    
Aspergillus oryzae MTCC 5341, when grown on wheat bran as substrate, produces several extracellular acid proteases. Production of the major acid protease (constituting 34% of the total) by solid-state fermentation is optimized. Optimum operating conditions obtained are determined as pH 5, temperature of incubation of 30 degrees C, defatted soy flour addition of 4%, and fermentation time of 120 h, resulting in acid protease production of 8.64 x 10(5) U/g bran. Response-surface methodology is used to generate a predictive model of the combined effects of independent variables such as, pH, temperature, defatted soy flour addition, and fermentation time. The statistical design indicates that all four independent variables have significant effects on acid protease production. Optimum factor levels are pH 5.4, incubation temperature of 31 degrees C, 4.4% defatted soy flour addition, and fermentation time of 123 h to yield a maximum activity of 8.93 x 10(5) U/g bran. Evaluation experiments, carried out to verify the predictions, reveal that A. oryzae produces 8.47 x 10(5) U/g bran, which corresponds to 94.8% of the predicted value. This is the highest acid protease activity reported so far, wherein the fungus produces four times higher activity than previously reported [J Bacteriol 130(1): 48-56, 1977].
K S Vishwanatha; A G Appu Rao; Sridevi Annapurna Singh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-25
Journal Detail:
Title:  Journal of industrial microbiology & biotechnology     Volume:  37     ISSN:  1476-5535     ISO Abbreviation:  J. Ind. Microbiol. Biotechnol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-04-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705544     Medline TA:  J Ind Microbiol Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  129-38     Citation Subset:  IM    
Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Mysore, India.
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MeSH Terms
Aspergillus oryzae / enzymology*
Carbon / metabolism
Culture Media
Hydrogen-Ion Concentration
Industrial Microbiology / methods*
Models, Statistical
Nitrogen / metabolism
Oryza sativa / metabolism
Peptide Hydrolases / biosynthesis*
Proteins / metabolism
Substrate Specificity
Time Factors
Triticum / metabolism
Reg. No./Substance:
0/Culture Media; 0/Proteins; 7440-44-0/Carbon; 7727-37-9/Nitrogen; EC 3.4.-/Peptide Hydrolases

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