Document Detail

Acid phosphatases do not contribute to the pathogenesis of type A Francisella tularensis.
MedLine Citation:
PMID:  19858304     Owner:  NLM     Status:  MEDLINE    
The intracellular pathogen Francisella tularensis is the causative agent of tularemia, a zoonosis that can affect humans with potentially lethal consequences. Essential to Francisella virulence is its ability to survive and proliferate within phagocytes through phagosomal escape and cytosolic replication. Francisella spp. encode a variety of acid phosphatases, whose roles in phagosomal escape and virulence have been documented yet remain controversial. Here we have examined in the highly virulent (type A) F. tularensis strain Schu S4 the pathogenic roles of three distinct acid phosphatases, AcpA, AcpB, and AcpC, that are most conserved between Francisella subspecies. Neither the deletion of acpA nor the combination of acpA, acpB, and acpC deletions affected the phagosomal escape or cytosolic growth of Schu S4 in murine and human macrophages, despite decreases in acid phosphatase activities by as much as 95%. Furthermore, none of these mutants were affected in their ability to cause lethality in mice upon intranasal inoculation. Hence, the acid phosphatases AcpA, AcpB, and AcpC do not contribute to intracellular pathogenesis and do not play a major role in the virulence of type A Francisella strains.
Robert Child; Tara D Wehrly; Dedeke Rockx-Brouwer; David W Dorward; Jean Celli
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2009-10-26
Journal Detail:
Title:  Infection and immunity     Volume:  78     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-23     Completed Date:  2010-01-21     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  59-67     Citation Subset:  IM    
Tularemia Pathogenesis Section, Laboratory of Intracellular Parasites, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, Hamilton, MT 59840, USA.
Data Bank Information
Bank Name/Acc. No.:
RefSeq/YP_169222;  YP_169276;  YP_169641;  YP_169785;  YP_170045;  YP_170416;  YP_897687;  YP_897755;  YP_898327;  YP_898596;  YP_898702;  YP_899174;  ZP_02274253;  ZP_02274312;  ZP_02274752;  ZP_02275294;  ZP_02275376;  ZP_02275415;  ZP_02275789;  ZP_02275790
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MeSH Terms
Acid Phosphatase / genetics,  metabolism*
Francisella tularensis / enzymology*,  genetics,  pathogenicity*
Gene Deletion
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Mice, Inbred BALB C
Tularemia / microbiology*
Reg. No./Substance:
EC Phosphatase

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