Document Detail


Acid phosphatases do not contribute to the pathogenesis of type A Francisella tularensis.
MedLine Citation:
PMID:  19858304     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intracellular pathogen Francisella tularensis is the causative agent of tularemia, a zoonosis that can affect humans with potentially lethal consequences. Essential to Francisella virulence is its ability to survive and proliferate within phagocytes through phagosomal escape and cytosolic replication. Francisella spp. encode a variety of acid phosphatases, whose roles in phagosomal escape and virulence have been documented yet remain controversial. Here we have examined in the highly virulent (type A) F. tularensis strain Schu S4 the pathogenic roles of three distinct acid phosphatases, AcpA, AcpB, and AcpC, that are most conserved between Francisella subspecies. Neither the deletion of acpA nor the combination of acpA, acpB, and acpC deletions affected the phagosomal escape or cytosolic growth of Schu S4 in murine and human macrophages, despite decreases in acid phosphatase activities by as much as 95%. Furthermore, none of these mutants were affected in their ability to cause lethality in mice upon intranasal inoculation. Hence, the acid phosphatases AcpA, AcpB, and AcpC do not contribute to intracellular pathogenesis and do not play a major role in the virulence of type A Francisella strains.
Authors:
Robert Child; Tara D Wehrly; Dedeke Rockx-Brouwer; David W Dorward; Jean Celli
Related Documents :
6375584 - Activities of proteinases and of a proteinase b inhibitor in tumors of the human uterus.
3742984 - Electrophoresis of human alkaline and acid phosphatases.
80054 - Counter immunoelectrophoresis for detection of human prostatic acid phosphatase.
17931864 - 2-aryl-3,3,3-trifluoro-2-hydroxypropionic acids: a new class of protein tyrosine phosph...
8930574 - Proton-pump inhibitors are the treatment of choice in acid-related disease.
18498024 - Combining txrf, ft-ir and gc-ms information for identification of inorganic and organic...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2009-10-26
Journal Detail:
Title:  Infection and immunity     Volume:  78     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-23     Completed Date:  2010-01-21     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  59-67     Citation Subset:  IM    
Affiliation:
Tularemia Pathogenesis Section, Laboratory of Intracellular Parasites, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, Hamilton, MT 59840, USA.
Data Bank Information
Bank Name/Acc. No.:
RefSeq/YP_169222;  YP_169276;  YP_169641;  YP_169785;  YP_170045;  YP_170416;  YP_897687;  YP_897755;  YP_898327;  YP_898596;  YP_898702;  YP_899174;  ZP_02274253;  ZP_02274312;  ZP_02274752;  ZP_02275294;  ZP_02275376;  ZP_02275415;  ZP_02275789;  ZP_02275790
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / genetics,  metabolism*
Animals
Francisella tularensis / enzymology*,  genetics,  pathogenicity*
Gene Deletion
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Mice
Mice, Inbred BALB C
Tularemia / microbiology*
Virulence
Chemical
Reg. No./Substance:
EC 3.1.3.2/Acid Phosphatase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Functional characterization of Borrelia spielmanii outer surface proteins that interact with distinc...
Next Document:  Division of Salmonella-Containing Vacuole and Depletion of Acidic Lysosomes in Salmonella-Infected H...