| Acid phosphatases do not contribute to the pathogenesis of type A Francisella tularensis. | |
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MedLine Citation:
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PMID: 19858304 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The intracellular pathogen Francisella tularensis is the causative agent of tularemia, a zoonosis that can affect humans with potentially lethal consequences. Essential to Francisella virulence is its ability to survive and proliferate within phagocytes through phagosomal escape and cytosolic replication. Francisella spp. encode a variety of acid phosphatases, whose roles in phagosomal escape and virulence have been documented yet remain controversial. Here we have examined in the highly virulent (type A) F. tularensis strain Schu S4 the pathogenic roles of three distinct acid phosphatases, AcpA, AcpB, and AcpC, that are most conserved between Francisella subspecies. Neither the deletion of acpA nor the combination of acpA, acpB, and acpC deletions affected the phagosomal escape or cytosolic growth of Schu S4 in murine and human macrophages, despite decreases in acid phosphatase activities by as much as 95%. Furthermore, none of these mutants were affected in their ability to cause lethality in mice upon intranasal inoculation. Hence, the acid phosphatases AcpA, AcpB, and AcpC do not contribute to intracellular pathogenesis and do not play a major role in the virulence of type A Francisella strains. |
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Authors:
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Robert Child; Tara D Wehrly; Dedeke Rockx-Brouwer; David W Dorward; Jean Celli |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural Date: 2009-10-26 |
Journal Detail:
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Title: Infection and immunity Volume: 78 ISSN: 1098-5522 ISO Abbreviation: Infect. Immun. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-23 Completed Date: 2010-01-21 Revised Date: 2010-09-28 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 59-67 Citation Subset: IM |
Affiliation:
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Tularemia Pathogenesis Section, Laboratory of Intracellular Parasites, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, Hamilton, MT 59840, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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RefSeq/YP_169222; YP_169276; YP_169641; YP_169785; YP_170045; YP_170416; YP_897687; YP_897755; YP_898327; YP_898596; YP_898702; YP_899174; ZP_02274253; ZP_02274312; ZP_02274752; ZP_02275294; ZP_02275376; ZP_02275415; ZP_02275789; ZP_02275790 |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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genetics,
metabolism* Animals Francisella tularensis / enzymology*, genetics, pathogenicity* Gene Deletion Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Mice Mice, Inbred BALB C Tularemia / microbiology* Virulence |
| Chemical | |
Reg. No./Substance:
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EC 3.1.3.2/Acid Phosphatase |
| Comments/Corrections | |
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