Document Detail


Acid phosphatase production by Rhizopus delemar: a role played in the Ni(II) bioaccumulation process.
MedLine Citation:
PMID:  20846788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The microbial growth and activity of acid phosphatase enzyme during the growth of Rhizopus delemar in the presence or absence of Ni(II) ions were investigated. An increase in initial Ni(II) ion concentration inhibited both growth rate of R. delemar and acid phosphatase activity. The maximum-intrinsic specific growth rate (μ(m)) and Monod constant (K(s)) of microorganism in Ni(II)-free medium were found as 0.0649 h(-1) and 1.8928 g/L, respectively. The inhibition of Ni(II) ions on growth rate of R. delemar was found to be a competitive inhibition and the inhibition constant was found to be 67.11 mg Ni(II)/L. The intrinsic Michaelis-Menten constant (K(m)) and maximum forward velocity of the reaction (v(m)) were determined as 3.17 mM and 833.3 μmol/L min, respectively, in Ni(II)-free medium. In the presence of Ni(II) ions, the activity of acid phosphatase was inhibited. Addition of Ni(II) ions decreased the maximum reaction velocity, v(m), showed noncompetitive-type inhibition kinetics and the inhibition constant was determined as 50mg Ni(II)/L. Maximum Ni(II) uptake was obtained by the growing cells of R. delemar, while the uptake capacity of resting cells was lowest. This study proved that acid phosphatase enzyme participated in the Ni(II) bioaccumulation mechanism of growing R. delemar.
Authors:
U Açikel; M Erşan
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Publication Detail:
Type:  Journal Article     Date:  2010-08-27
Journal Detail:
Title:  Journal of hazardous materials     Volume:  184     ISSN:  1873-3336     ISO Abbreviation:  J. Hazard. Mater.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422688     Medline TA:  J Hazard Mater     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  632-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Chemical Engineering Department, Cumhuriyet University, 58140 Sivas, Turkey. unsal.acikel@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / biosynthesis*
Nickel / metabolism*
Rhizopus / enzymology*,  metabolism
Soil Pollutants / metabolism*
Chemical
Reg. No./Substance:
0/Soil Pollutants; 7440-02-0/Nickel; EC 3.1.3.2/Acid Phosphatase

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