Document Detail


Acid phosphatase activity during the interaction of the nematophagous fungus Duddingtonia flagrans with the nematode Panagrellus sp.
MedLine Citation:
PMID:  19679133     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of Duddingtonia flagrans, a nematode-trapping fungus, has been investigated as a biological control method against free living larvae of gastrointestinal nematodes of livestock animals. This fungus captures and infects the nematode by cuticle penetration, immobilization and digestion of the internal contents. It has been suggested that this sequence of events occurs by a combination of physical and enzymatical activities. This report characterizes the acid phosphatase activity during the interaction of D. flagrans with the free-living nematode Panagrellus sp. The optimum pH for the hydrolysis of the acid phosphatase substrate p-nitrophenyl phosphate was 2.2, 2.8 and 5.4 from D. flagrans alone and 2.2 and 5.4 for Panagrellus sp alone, fungus-nematode interaction in liquid medium and fungus-nematode interaction in solid medium. Different acid phosphatase activity bands were detected by SDS-PAGE. Maximum acid phosphatase activity of the fungus or nematode alone and of the fungus-nematode interaction occurred within 70min of incubation in the presence of the substrate 4-methylumbelliferyl phosphate. The activity of this enzyme was significantly higher for the fungus-nematode interaction when compared to the organisms alone, indicating a synergistic response. Furthermore, structures appeared in the hyphae after 30min, nematodes were observed adhered after 40min and many were captured by the typical fungus traps after 70min of interaction. The participation of acid phosphatase activity and its importance during the interaction of the fungus with the nematode were discussed.
Authors:
D G Cruz; C P Silva; C N B Carneiro; C A Retamal; J T L Thi??baut; R A DaMatta; C P Santos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-11
Journal Detail:
Title:  Journal of invertebrate pathology     Volume:  102     ISSN:  1096-0805     ISO Abbreviation:  J. Invertebr. Pathol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-02     Completed Date:  2010-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014067     Medline TA:  J Invertebr Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  238-44     Citation Subset:  IM    
Affiliation:
Laborat??rio de Biologia Celular e Tecidual, Centro de Bioci??ncias e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / metabolism*,  physiology
Animals
Ascomycota / enzymology*
Electrophoresis, Polyacrylamide Gel
Hydrogen-Ion Concentration
Kinetics
Rhabditida / enzymology,  microbiology*
Time Factors
Chemical
Reg. No./Substance:
EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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