Document Detail

Acid-induced COX-2 expression and prostaglandin E(2) production via activation of ERK1/2 and p38 MAPK in cultured feline esophageal smooth muscle cells.
MedLine Citation:
PMID:  22210040     Owner:  NLM     Status:  In-Data-Review    
Cyclooxygenase (COX)-2 is known to play an important role in inflammatory conditions such as reflux esophagitis resulting from acid reflux. In this study, we tested whether an acidic medium (pH 4.0) induces an increase in COX-2 expression or PGE(2) production, and explored the implication of mitogen-activated protein kinases (MAPKs) activation in these responses in cultured cat esophageal smooth muscle cells. Acidic cytotoxicity was assessed and expression changes in COXs or phosphorylated MAPKs were analyzed by Western blotting. PGE(2) production was measured by immunoassay. No significant decrease in cell viability was observed for 6 h exposure to acidic medium. COX-2 expression and PGE(2) production significantly increased to maximal levels at 6 h exposure to acidic medium. The cells also exhibited significant activation of ERK1/2 and p38 MAPK, but not JNK within 10 min under acidic medium. The increments of COX-2 expression and PGE(2) production by acidic medium were decreased by pretreatment with PD98059 or SB202190, respectively. These results suggest that acidic environments may enhance the COX-2 expression and PGE(2) production through activation of ERK1/2 and p38 MAPK in the cultured cat esophageal smooth muscle cells.
Jae Gwang Woo; Sun Young Park; Jae Chun Lim; Min-Jae Joo; Hak Rim Kim; Uy Dong Sohn
Publication Detail:
Type:  Journal Article     Date:  2011-12-31
Journal Detail:
Title:  Archives of pharmacal research     Volume:  34     ISSN:  0253-6269     ISO Abbreviation:  Arch. Pharm. Res.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-01-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000036     Medline TA:  Arch Pharm Res     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  2131-40     Citation Subset:  IM    
Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Korea.
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