Document Detail

Acid hydrolases in blister fluid. I. Characterization and quantification of acid phosphatase.
MedLine Citation:
PMID:  25078     Owner:  NLM     Status:  MEDLINE    
Acid phosphatase has been characterized and quantified in human suction blister fluid, interstitial fluid and serum. The acid phosphatases of suction blister fluid and serum showed differences in their pH activity curves, Michaelis-Menton constants, heat stabilities and sensitivities to inhibition by tartrate and fluoride. The behaviour of the interstitial fluid enzyme was intermediate between these two. The levels of activity in blister fluid and serum were very similar, both being more than twice that of interstitial fluid. These results suggest that the acid phosphatase activity in suction blister fluid is derived largely from the overlying epidermis rather than from serum.
G Volden
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The British journal of dermatology     Volume:  98     ISSN:  0007-0963     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  1978 Mar 
Date Detail:
Created Date:  1978-06-17     Completed Date:  1978-06-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  273-80     Citation Subset:  IM    
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MeSH Terms
Acid Phosphatase / antagonists & inhibitors,  blood,  metabolism*
Blister / enzymology*
Extracellular Space / enzymology
Hot Temperature
Hydrogen-Ion Concentration
Proteins / analysis
Time Factors
Reg. No./Substance:
0/Proteins; EC Phosphatase

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