Document Detail

Acid glycohydrolase in Chinese hamster with spontaneous diabetes. I. Depressed levels of renal alpha-galactosidase and beta-galactosidase.
MedLine Citation:
PMID:  23847     Owner:  NLM     Status:  MEDLINE    
The activites of alpha-and Beta-galactosidases (alpha-D-galactoside galactohydrolase, EC; beta-D-galactoside galactohydrolase, EC were significantly lower in the kidneys of diabetic XA line than those in the nondiabetic M line Chinese hamsters. The depression of these enzymes was found only in the kidney but not in liver, spleen, hind leg muscle, cheek pouch or spinal cord. In young XA animals before onset of glycosuria, renal alpha-galactosidase level was similar to that in age-matched M animals; whereas, their renal beta-galactosidase activity was about 90% of those in the M animals. Partial purification and separation of these enzymes were achieved by chromatography on DEAE-Sepharose CL-6B columns. beta-galactosidase was separated into two isozymes and depression of activity in the XA kidneys was evident in both. alpha-galactosidase was recovered in a single peak. The pH optima of these enzymes from XA and M animals were identical. With p-nitrophenyl glycosides as substrates, the Michaelis constants of these enzymes were also the same of XA and M animals. Molecular weight estimation by gel filtration on Sepharose 6B yielded similar results between M and XA samples: 2.4-10(5) for alpha-galactosidase and 1.6.10(5) and 1.9.10(5) for beta-galactosidase isozymes. The data suggest that the diabetic animals had lower concentrations of alpha-and beta-galactosidase in their kidneys, probably as a consequence of hyperglycemia.
A Y Chang
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  522     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1978 Feb 
Date Detail:
Created Date:  1978-04-17     Completed Date:  1978-04-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  491-502     Citation Subset:  IM    
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MeSH Terms
Diabetes Mellitus / enzymology*,  genetics
Galactosidases / metabolism*
Hydrogen-Ion Concentration
Kidney / enzymology
Molecular Weight
Polyethylene Glycols / pharmacology
Tissue Distribution
alpha-Galactosidase / metabolism*
beta-Galactosidase / metabolism*
Reg. No./Substance:
0/Polyethylene Glycols; EC 3.2.1.-/Galactosidases; EC; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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