| Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension. | |
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MedLine Citation:
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PMID: 21400674 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation. |
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Authors:
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Robin Treuer; Shelley E Haydel |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Current protocols in microbiology Volume: Chapter 10 ISSN: 1934-8533 ISO Abbreviation: Curr Protoc Microbiol Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-03-14 Completed Date: 2011-07-12 Revised Date: 2013-05-24 |
Medline Journal Info:
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Nlm Unique ID: 101257113 Medline TA: Curr Protoc Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: Unit 10A.6 Citation Subset: IM |
Copyright Information:
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© 2011 by John Wiley & Sons, Inc. |
Affiliation:
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School of Life Sciences, Arizona State University, Tempe, Arizona, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriological Techniques
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methods* Coloring Agents / chemistry Containment of Biohazards Mycobacterium / chemistry*, cytology* Staining and Labeling / methods* |
| Grant Support | |
ID/Acronym/Agency:
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AT004690/AT/NCCAM NIH HHS; R01 AT004690/AT/NCCAM NIH HHS; R01 AT004690-02/AT/NCCAM NIH HHS; R01 AT004690-04/AT/NCCAM NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Coloring Agents |
| Comments/Corrections | |
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