Document Detail


Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension.
MedLine Citation:
PMID:  21400674     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation.
Authors:
Robin Treuer; Shelley E Haydel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Current protocols in microbiology     Volume:  Chapter 10     ISSN:  1934-8533     ISO Abbreviation:  Curr Protoc Microbiol     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-03-14     Completed Date:  2011-07-12     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101257113     Medline TA:  Curr Protoc Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 10A.6     Citation Subset:  IM    
Copyright Information:
© 2011 by John Wiley & Sons, Inc.
Affiliation:
School of Life Sciences, Arizona State University, Tempe, Arizona, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques / methods*
Coloring Agents / chemistry
Containment of Biohazards
Mycobacterium / chemistry*,  cytology*
Staining and Labeling / methods*
Grant Support
ID/Acronym/Agency:
AT004690/AT/NCCAM NIH HHS; R01 AT004690/AT/NCCAM NIH HHS; R01 AT004690-02/AT/NCCAM NIH HHS; R01 AT004690-04/AT/NCCAM NIH HHS
Chemical
Reg. No./Substance:
0/Coloring Agents
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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