Document Detail

Acid-fast bodies in faecal smears stained by the modified Ziehl-Neelsen technique.
MedLine Citation:
PMID:  11284227     Owner:  NLM     Status:  MEDLINE    
The modified Ziehl-Neelsen (ZN) stain has proved useful in the laboratory diagnosis of cryptosporidiosis and, more recently, for the laboratory diagnosis of cyclospora. Apart from cryptosporidia and cyclospora, many other organisms and artefacts are present in faeces, and may be seen in faecal smears stained by the modified ZN method. Described here is the presence of such organisms and artefacts in faecal samples submitted to the routine microbiology laboratory of a district general hospital. Over 6000 faecal smears were examined using the modified ZN method, with an incidence of cyclospora and cryptosporidium of approximately 0.1% and 1%, respectively. Other organisms and artefacts were observed, with an incidence ranging from 0.1% to 1%. It is emphasised that the identification of known gastrointestinal tract pathogens should not rely solely on the results of their staining reactions. It is essential that criteria such as morphology and size be taken into account to differentiate organisms with similar staining reactions.
S C Clarke; M McIntyre
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of biomedical science     Volume:  58     ISSN:  0967-4845     ISO Abbreviation:  Br. J. Biomed. Sci.     Publication Date:  2001  
Date Detail:
Created Date:  2001-04-03     Completed Date:  2001-04-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9309208     Medline TA:  Br J Biomed Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  7-10     Citation Subset:  IM    
Scottish Meningococcus and Pneumococcus Reference Laboratory, Department of Microbiology, House on the Hill, Stobhill Hospital, Glasgow G21 3UW, UK.
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MeSH Terms
Algae, Red / isolation & purification
Cryptosporidiosis / diagnosis
Cryptosporidium / isolation & purification
Cyclospora / isolation & purification*
Cyclosporiasis / diagnosis
Feces / microbiology*
Staining and Labeling / methods*

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