Document Detail


Acid cleavable surface enhanced raman tagging for protein detection.
MedLine Citation:
PMID:  21109888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dye conjugation is a common strategy improving the surface enhanced Raman detection sensitivity of biomolecules. Reported is a proof-of-concept study of a novel surface enhanced Raman spectroscopic tagging strategy termed as acid-cleavable SERS tag (ACST) method. Using Rhodamine B as the starting material, we prepared the first ACST prototype that consisted of, from the distal end, a SERS tag moiety (STM), an acid-cleavable linker, and a protein reactive moiety. Complete acid cleavage of the ACST tags was achieved at a very mild condition that is 1.5% trifluoroacetic acid (TFA) aqueous solution at room temperature. SERS detection of this ACST tagged protein was demonstrated using bovine serum albumin (BSA) as the model protein. While the SERS spectrum of intact ACST-BSA was entirely dominated by the fluorescent signal of STM, quality SERS spectra can be readily obtained with the acid cleaved ACST-BSA conjugates. Separation of the acid cleaved STM from protein further enhances the SERS sensitivity. Current SERS detection sensitivity, achieved with the acid cleaved ACST-BSA conjugate is ∼5 nM in terms of the BSA concentration and ∼1.5 nM in ACST content. The dynamic range of the cleaved ACST-BSA conjugate spans four orders of magnitudes from ∼10 nM to ∼100 μM in protein concentrations. Further improvement in the SERS sensitivity can be achieved with resonance Raman acquisition. This cleavable tagging strategy may also be used for elimination of protein interference in fluorescence based biomolecule detection.
Authors:
Dongmao Zhang; Karthikeshwar Vangala; Shaoyong Li; Michael Yanney; Hao Xia; Sige Zou; Andrzej Sygula
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2010-11-26
Journal Detail:
Title:  The Analyst     Volume:  136     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-19     Completed Date:  2011-05-06     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  520-6     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Mississippi State University, Mississippi State, MS 39762, USA. dz33@msstate.edu
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MeSH Terms
Descriptor/Qualifier:
Electrophoresis, Polyacrylamide Gel
Fluorescent Dyes / chemistry*
Molecular Structure
Proteins / analysis*
Rhodamines / chemistry*
Sensitivity and Specificity
Serum Albumin, Bovine / analysis
Spectrum Analysis, Raman / methods*
Substrate Specificity
Trifluoroacetic Acid / chemistry*
Grant Support
ID/Acronym/Agency:
ZIA AG000365-06/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Proteins; 0/Rhodamines; 0/Serum Albumin, Bovine; 76-05-1/Trifluoroacetic Acid; K7G5SCF8IL/rhodamine B
Comments/Corrections

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