| Acid-base properties of the nucleic-acid model 2'-deoxyguanylyl(5'-->3')-2'-deoxy-5'-guanylate, d(pGpG)3-, and of related guanine derivatives. | |
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MedLine Citation:
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PMID: 16525552 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The dinucleotide d(pGpG) is an often employed DNA model to study various kinds of interactions between DNA and metal ions, but its acid-base properties were not yet described in detail. In this study the six deprotonation reactions of H4[d(pGpG)]+ are quantified. The acidity constants for the release of the first proton from the terminal P(O)(OH)2 group (pKa = 0.65) and for one of the (N7)H+ sites (pKa = 2.4) are estimated. The acidity constants of the remaining four deprotonation reactions were measured by potentiometric pH titrations in aqueous solution (25 degrees C; I = 0.1 M, NaNO3): The pKa values for the deprotonations of the second (N7)H+, the P(O)2(OH)-, and the two (N1)H sites are 2.98, 6.56, 9.54 and 10.11, respectively. Based on these results we show how to estimate acidity constants for related systems that have not been studied, e.g. pGpG, which is involved in the initiation step of a rotavirus RNA polymerase. The relevance of our results for nucleic acids in general is briefly indicated. |
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Authors:
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Bernd Knobloch; Helmut Sigel; Andrzej Okruszek; Roland K O Sigel |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-26 |
Journal Detail:
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Title: Organic & biomolecular chemistry Volume: 4 ISSN: 1477-0520 ISO Abbreviation: Org. Biomol. Chem. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-03-09 Completed Date: 2007-09-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101154995 Medline TA: Org Biomol Chem Country: England |
Other Details:
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Languages: eng Pagination: 1085-90 Citation Subset: IM |
Affiliation:
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Institute of Inorganic Chemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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5'-Guanylic Acid
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chemistry Deoxyguanine Nucleotides / chemistry* Dideoxynucleosides / chemistry* Guanine / analogs & derivatives*, chemistry* Hydrogen-Ion Concentration* Kinetics Molecular Conformation Uridine Monophosphate / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Deoxyguanine Nucleotides; 0/Dideoxynucleosides; 58-97-9/Uridine Monophosphate; 73-40-5/Guanine; 85-32-5/5'-Guanylic Acid; 902-04-5/2'-deoxyguanosine 5'-phosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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