| Acid and Neutral Invertases in the Mesocarp of Developing Muskmelon (Cucumis melo L. cv Prince) Fruit. | |
| | |
MedLine Citation:
|
PMID: 16668269 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
Acid and neutral invertases were found in the mesocarp of developing muskmelon (Cucumis melo L. cv Prince) fruit and the activities of these enzymes declined with maturation of the fruit, concomitantly with the accumulation of sucrose. Neutral invertase was only present in the soluble fraction and acid invertase was present in both the soluble and cell-wall fractions. The cell-wall fraction contained three types of acid invertase: a NaCl-released invertase; an EDTA-released invertase, and a tightly bound invertase that still remained on the cell wall after treatment with NaCl and EDTA. The soluble acid and neutral invertases could be separated from one another by chromatography on DEAE-cellulose and they exhibited clear differences in their properties, namely, in their pH optima, substrate specificity, K(m) values for sucrose, and inhibition by metal ions. The EDTA-released invertase and the soluble acid invertase were similar with regard to their chromatographic behavior on DEAE-cellulose, but the NaCl-released invertase was different because it was adsorbed to a column of CM-cellulose. The soluble acid invertase and two cell-wall bound invertases had very similar characteristics with regard to optimal pH and temperature, K(m) value for sucrose, and substrate specificity. |
| | |
Authors:
|
A P Ranwala; S S Iwanami; H Masuda |
Related Documents
:
|
11791709 - Structural characterization and immunomodulating activity of a complex glucan from spor... 15868209 - Immunocytochemistry of pectins in shoot apical meristems: consequences for intercellula... 23095419 - Acid-base and electrolyte abnormalities during renal support for acute kidney injury: r... 417919 - Further evidence for the structure of the teichoic acids from bacillus stearothermophil... 46329 - Polysaccharides of metaplastic mucosa and carcinoma of the gallbladder. 20615429 - Structural characterization of polysaccharides from zizyphus jujuba and evaluation of a... 9472789 - 13c-cp-mas-nmr studies of flavonoids. i. solid-state conformation of quercetin, quercet... 7519339 - Evidence for a regional distribution of hyaluronic acid in the rat brain using a highly... 148279 - Heterogeneity in the composition and aggregation patterns of coronary intima acid mucop... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Plant physiology Volume: 96 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1991 Jul |
Date Detail:
|
Created Date: 2010-06-29 Completed Date: 2010-06-29 Revised Date: 2010-09-15 |
Medline Journal Info:
|
Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: 881-6 Citation Subset: - |
Affiliation:
|
Department of Agricultural Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Biochemical Bases for the Loss of Basipetal IAA Transport with Advancing Physiological Age in Etiola...
Next Document: Sucrose Synthase Expression during Cold Acclimation in Wheat.