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Acid-Mediated Prevention of Aspartimide Formation in Solid Phase Peptide Synthesis.
MedLine Citation:
PMID:  23025410     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aspartimide formation is one of the major obstacles that impedes the solid phase synthesis of large peptides and proteins. Until now, no cost-effective strategy to suppress this side reaction has been developed. Here it is demonstrated that addition of small amounts of organic acids to the standard Fmoc cleavage agent piperidine efficiently prevents formation of aspartimide side products. This effect is shown to be virtually independent of the acid strength.
Authors:
Tillmann Michels; Rudolf Dölling; Uwe Haberkorn; Walter Mier
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-1
Journal Detail:
Title:  Organic letters     Volume:  -     ISSN:  1523-7052     ISO Abbreviation:  Org. Lett.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100890393     Medline TA:  Org Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Nuclear Medicine, University Hospital Heidelberg , INF 400, 69120 Heidelberg, Germany, and BIOSYNTAN GmbH , Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
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