Document Detail


Acid-labile liposome/pDNA complexes.
MedLine Citation:
PMID:  20072897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One of the bottlenecks to achieve gene or drug delivery to cells is the spatio-temporal release of the cargo to effect the correct task wherever and whenever it is supposed to. The aim of this chapter is to describe the synthesis and properties of lipids, which can form liposome complexes with DNA and to fall apart in slight acidic condition such as those encountered in the late endosome, thus destabilizing the liposome membrane and releasing their content.
Authors:
Michel Bessodes; Daniel Scherman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  605     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-01-14     Completed Date:  2010-04-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  405-23     Citation Subset:  IM    
Affiliation:
Unit? de Pharmacologie Chimique et G?n?tique, Inserm, U640, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Acids / chemistry
DNA / administration & dosage*
Genes, Reporter
Hela Cells
Humans
Hydrogen-Ion Concentration
Lipids / chemical synthesis,  chemistry*
Liposomes / chemistry*
Molecular Structure
Plasmids / administration & dosage
Transfection*
Chemical
Reg. No./Substance:
0/Acids; 0/Lipids; 0/Liposomes; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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