Document Detail


Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics.
MedLine Citation:
PMID:  21539393     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.
Authors:
Jessica L Cohen; Stephanie Schubert; Peter R Wich; Lina Cui; Joel A Cohen; Justin L Mynar; Jean M J Fréchet
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-05-24
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  22     ISSN:  1520-4812     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-15     Completed Date:  2011-11-02     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1056-65     Citation Subset:  IM    
Affiliation:
College of Chemistry, University of California, Berkeley, California 94720-1460, United States.
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MeSH Terms
Descriptor/Qualifier:
Acids / chemistry*
Carbohydrate Conformation
Cations / chemical synthesis,  chemistry
Cell Survival / drug effects
Cells, Cultured
Dextrans / chemical synthesis,  chemistry*,  pharmacology
Dose-Response Relationship, Drug
Drug Delivery Systems / methods*
HeLa Cells
Humans
Hydrogen-Ion Concentration
Particle Size
RNA, Small Interfering / administration & dosage*,  pharmacology
Structure-Activity Relationship
Surface Properties
Transfection / methods*
Grant Support
ID/Acronym/Agency:
HHSN268201000043C//PHS HHS; HHSN268201000046C/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Acids; 0/Cations; 0/RNA, Small Interfering; 9004-54-0/Dextrans
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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