| Acid and Base Secretion in Freshly Excised Nasal Tissue From Cystic Fibrosis Patients with ΔF508 Mutation. | |
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MedLine Citation:
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PMID: 22034590 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Cystic fibrosis (CF) is caused by a misfunctional CFTR protein, which is believed to contributes to the regulation of the airway surface liquid (ASL) pH. This study investigated acid and base secretion in freshly excised human nasal tissues from CF patients homozygous for the ΔF508 mutation. Human nasal mucosa was collected during sinus surgery and investigated in Ussing chambers. Mucosal equilibrium pH values and rate of acid and base secretion were determined using the pH-stat technique. The equilibrium pH of nasal epithelia from ΔF508 CF patients with chronic rhinosinusitis (CRS) was pH = 7.08 ± 0.09 and significantly lower compared to nasal epithelia from CRS without CF (pH = 7.33 ± 0.06) and normal subjects (pH = 7.34 ± 0.08, n = 6). The rate of base secretion in CF nasal tissues was 11.8 ± 2.4 nmol·min(-1)·cm(-2), which was significantly lower than normal (57.2 ± 9.2 nmol·min(-1)·cm(-2)). HCO(3) (-)secretory rate was further increased by forskolin by 16.1% in normal, but not in CF tissues. Our data suggests that CF patients exhibited significantly lower base secretion by the nasal airway epithelium. It is possible that improper regulation of ASL pH in CF may negatively impact the innate host defense system. |
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Authors:
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Do-Yeon Cho; Peter H Hwang; Beate Illek; Horst Fischer |
Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: International forum of allergy & rhinology Volume: 1 ISSN: 2042-6984 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-10-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101550261 Medline TA: Int Forum Allergy Rhinol Country: - |
Other Details:
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Languages: ENG Pagination: 123-127 Citation Subset: - |
Affiliation:
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Division of Rhinology, Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, California 94305-5739. |
Export Citation:
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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R01 HL086323-04//NHLBI NIH HHS; R21 HL089196-02//NHLBI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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