Document Detail

Achieving high survival rate following cryopreservation after isolation of prepubertal mouse spermatogonial cells.
MedLine Citation:
PMID:  19199023     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Isolating spermatogonia cells with high purity and viability and achieving better survival rate following cryopreservation METHODS: Isolating the cells by Magnetic Activating Cell Sorting (MACS) method using anti CD49f (alpha6 integrin) antibody and Dynabeads and freezing in DMSO-based freezing mediums containing three different FBS concentrations of 50%, 60% and 70%. RESULTS: The mean (+/-SD) purity of the isolated cells was 92.52+/-3.57 (range 92.43-98.25). The cells frozen in group I, II and III had mean 39.60+/-1.48 (range 37.98-41.62), 89.05+/-3.83 (range 80.83-90.33) and 90.52+/-1.71 (range 89.07-92.52) viability, respectively. CONCLUSION: Higher viable cell counts and purity can be attained by the use of alpha6 integrin and magnetic beads. After the thawing of spermatogonial cells, optimum viability was achieved in freezing media containing 60% FBS.
Firooz Jannat Alipoor; Mohammad Ali Sadighi Gilani; Poopak Eftekhari-Yazdi; Ali Daliri Hampa; Hani Hosseinifar; Hiva Alipour; Mehdi Lotfi Panah
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Publication Detail:
Type:  Journal Article     Date:  2009-02-07
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  26     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-13     Completed Date:  2009-05-26     Revised Date:  2010-09-23    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  143-9     Citation Subset:  IM    
Royan Institute,Andrology Lab, P.O.Box: 19395-4644, Tehran, Iran.
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MeSH Terms
Cell Survival*
Flow Cytometry
Immunomagnetic Separation
Reproductive Techniques, Assisted
Sexual Maturation
Spermatogonia* / physiology,  transplantation

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