Document Detail

Achievements and perspectives of top-down proteomics.
MedLine Citation:
PMID:  20859958     Owner:  NLM     Status:  MEDLINE    
Over the last years, top-down (TD) MS has gained a remarkable space in proteomics, rapidly trespassing the limit between a promising approach and a solid, established technique. Several research groups worldwide have implemented TD analysis in their routine work on proteomics, deriving structural information on proteins with the level of accuracy that is impossible to achieve with classical bottom-up approaches. Complete maps of PTMs and assessment of single aminoacid polymorphisms are only a few of the results that can be obtained with this technique. Despite some existing technical and economical limitations, TD analysis is at present the most powerful instrument for MS-based proteomics and its implementation in routine workflow is a rapidly approaching turning point in proteomics. In this review article, the state-of-the-art of TD approach is described along with its major advantages and drawbacks and the most recent trends in TD analysis are discussed. References for all the covered topics are reported in the text, with the aim to support both newcomers and mass spectrometrists already introduced to TD proteomics.
Andrea Armirotti; Gianluca Damonte
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Publication Detail:
Type:  Historical Article; Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Proteomics     Volume:  10     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-14     Completed Date:  2011-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3566-76     Citation Subset:  IM    
Advanced Biotechnology Center, Largo Rosanna Benzi, Genova, Italy.
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MeSH Terms
Amino Acid Sequence
History, 21st Century
Mass Spectrometry / instrumentation,  methods*
Molecular Sequence Data
Proteins / analysis*,  genetics,  isolation & purification
Proteomics / history,  instrumentation,  methods*
Reg. No./Substance:

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