Document Detail


Acetylecholinesterase-based biosensor electrodes for organophosphate pesticide detection. II. Immobilization and stabilization of acetylecholinesterase.
MedLine Citation:
PMID:  15797334     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The dry and wet stability of Drosophila acetylcholinesterase non-covalently immobilized onto polyethyleneimine modified screen-printed carbon electrodes was improved when compared to non-immobilized acetylcholinesterase, and acetylcholinesterase covalently immobilized onto dialdehyde and polyethyleneimine modified electrodes. Stabilizer mixtures were characterized for additional stabilization of acetylcholinesterase during storage in the dry state, with dextran-sulphate/sucrose and polygalacturonic acid/sucrose mixtures proving highly effective for long-term storage of biosensor electrodes.
Authors:
A Vakurov; C E Simpson; C L Daly; T D Gibson; P A Millner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  20     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-03-30     Completed Date:  2005-08-01     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  2324-9     Citation Subset:  IM    
Affiliation:
School of Biochemistry and Microbiology, University of Leeds, Leeds, LS2 9JT, UK.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / analysis,  chemistry*
Biosensing Techniques / instrumentation,  methods*
Coated Materials, Biocompatible / analysis,  chemistry*
Electrochemistry / instrumentation,  methods*
Environmental Monitoring / instrumentation,  methods*
Enzyme Activation
Enzyme Stability
Enzymes, Immobilized / analysis,  chemistry
Equipment Design
Equipment Failure Analysis
Materials Testing
Pesticides / analysis*
Phosphoric Acid Esters / analysis*
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Enzymes, Immobilized; 0/Pesticides; 0/Phosphoric Acid Esters; EC 3.1.1.7/Acetylcholinesterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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