Document Detail

Acetylcholinesterase-polyaniline biosensor investigation of organophosphate pesticides in selected organic solvents.
MedLine Citation:
PMID:  17454383     Owner:  NLM     Status:  MEDLINE    
The behavior of an amperometric organic-phase biosensor consisting of a gold electrode modified first with a mercaptobenzothiazole self-assembled monolayer, followed by electropolymerization of polyaniline in which acetylcholinesterase as enzyme was immobilized, has been developed and evaluated for organophosphorous pesticide detection. The voltammetric results have shown that the formal potential shifts anodically as the Au/MBT/PANI/AChE/PVAc thick-film biosensor responded to acetylthiocholine substrate addition under anaerobic conditions in selected organic solvent media containing 2% v/v 0.05 M phosphate buffer, 0.1 M KCl (pH 7.2) solution. Detection limits in the order of 0.147 ppb for diazinon and 0.172 ppb for fenthion in acetone-saline phosphate buffer solution, and 0.180 ppb for diazinon and 0.194 ppb for fenthion in ethanol-saline phosphate buffer solution has been achieved.
Vernon S Somerset; Michael J Klink; Priscilla G L Baker; Emmanuel I Iwuoha
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes     Volume:  42     ISSN:  0360-1234     ISO Abbreviation:  J Environ Sci Health B     Publication Date:    2007 Mar-Apr
Date Detail:
Created Date:  2007-04-24     Completed Date:  2007-06-18     Revised Date:  2009-07-21    
Medline Journal Info:
Nlm Unique ID:  7607167     Medline TA:  J Environ Sci Health B     Country:  United States    
Other Details:
Languages:  eng     Pagination:  297-304     Citation Subset:  IM    
Department of Chemistry, University of the Western Cape, Bellville, South Africa.
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MeSH Terms
Acetylcholinesterase / chemistry*
Aniline Compounds / chemistry
Biosensing Techniques / methods
Diazinon / chemistry*
Enzymes, Immobilized / chemistry
Fenthion / chemistry*
Hydrogen-Ion Concentration
Organophosphorus Compounds / analysis,  chemistry
Pesticides / analysis,  chemistry*
Reg. No./Substance:
0/Aniline Compounds; 0/Enzymes, Immobilized; 0/Organophosphorus Compounds; 0/Pesticides; 0/Solvents; 0/polyaniline; 333-41-5/Diazinon; 55-38-9/Fenthion; 7440-57-5/Gold; EC

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