| Acetylcholinesterase-polyaniline biosensor investigation of organophosphate pesticides in selected organic solvents. | |
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MedLine Citation:
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PMID: 17454383 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The behavior of an amperometric organic-phase biosensor consisting of a gold electrode modified first with a mercaptobenzothiazole self-assembled monolayer, followed by electropolymerization of polyaniline in which acetylcholinesterase as enzyme was immobilized, has been developed and evaluated for organophosphorous pesticide detection. The voltammetric results have shown that the formal potential shifts anodically as the Au/MBT/PANI/AChE/PVAc thick-film biosensor responded to acetylthiocholine substrate addition under anaerobic conditions in selected organic solvent media containing 2% v/v 0.05 M phosphate buffer, 0.1 M KCl (pH 7.2) solution. Detection limits in the order of 0.147 ppb for diazinon and 0.172 ppb for fenthion in acetone-saline phosphate buffer solution, and 0.180 ppb for diazinon and 0.194 ppb for fenthion in ethanol-saline phosphate buffer solution has been achieved. |
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Authors:
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Vernon S Somerset; Michael J Klink; Priscilla G L Baker; Emmanuel I Iwuoha |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes Volume: 42 ISSN: 0360-1234 ISO Abbreviation: J Environ Sci Health B Publication Date: 2007 Mar-Apr |
Date Detail:
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Created Date: 2007-04-24 Completed Date: 2007-06-18 Revised Date: 2009-07-21 |
Medline Journal Info:
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Nlm Unique ID: 7607167 Medline TA: J Environ Sci Health B Country: United States |
Other Details:
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Languages: eng Pagination: 297-304 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of the Western Cape, Bellville, South Africa. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholinesterase
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chemistry* Anaerobiosis Aniline Compounds / chemistry Biosensing Techniques / methods Diazinon / chemistry* Electrochemistry Enzymes, Immobilized / chemistry Fenthion / chemistry* Gold Hydrogen-Ion Concentration Organophosphorus Compounds / analysis, chemistry Oxidation-Reduction Pesticides / analysis, chemistry* Solvents |
| Chemical | |
Reg. No./Substance:
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0/Aniline Compounds; 0/Enzymes, Immobilized; 0/Organophosphorus Compounds; 0/Pesticides; 0/Solvents; 0/polyaniline; 333-41-5/Diazinon; 55-38-9/Fenthion; 7440-57-5/Gold; EC 3.1.1.7/Acetylcholinesterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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