Document Detail

Acetylcholinesterase inhibitor acting on the brain improves detrusor overactivity caused by cerebral infarction in rats.
MedLine Citation:
PMID:  16905267     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The functional contribution of the cholinergic pathway in the frontal cortex to micturition was evaluated following cerebral ischemia. Furthermore, it was examined whether reactivation of this regulatory system using acetylcholinesterase inhibitor could improve detrusor overactivity. METHODS: Left middle cerebral artery occlusion (MCAO) was performed in female Sprague-Dawley rats. Choline acetyltransferase (ChAT) activities after MCAO were assayed to assess the damage to cholinergic neurons. ChAT activities in the bilateral cortex, hippocampus, and pons were calculated by measuring the conversion of 1-[14C] acetyl-coenzyme A to [14C] acetylcholine. Effects on cystometrography of i.v. or i.c.v. donepezil hydrochloride (DON), a centrally acting acetylcholinesterase inhibitor, were investigated in conscious sham-operated (SO) and cerebral infarcted (CI) rats. To investigate whether DON in the forebrain was affected, we decerebrated rats after CI or SO, and investigated the effects on cystometrography of i.v. DON. RESULTS: Bladder capacity was markedly decreased after MCAO, and remained below half of the pre-occlusion capacity. The greatest increase in bladder capacity was attained at 1.2 x 10(-2) nM/kg of DON given i.v., with a change of 52.8% (P < 0.05). In cases of i.c.v. DON, the greatest increase in bladder capacity was at the dose of 6 x 10(-2) pmol with the change of 95.8% (P < 0.01). The activity of ChAT was decreased in the left cortex and hippocampus 24 h after MCAO (P < 0.05). In decerebrated rats, low dose of DON did not change micturition parameters. CONCLUSIONS: These results suggest that by upregulation of the forebrain muscarinic inhibitory mechanism, acetylcholinesterase inhibitor improves detrusor overactivity by cerebral infarction.
M Nakai; H Akino; T Kaneda; Y Matsuta; R Shiyama; K Tanase; H Ito; Y Aoki; N Oyama; Y Miwa; O Yokoyama
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2006-08-14
Journal Detail:
Title:  Neuroscience     Volume:  142     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-25     Completed Date:  2007-01-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  475-80     Citation Subset:  IM    
Department of Urology, University of Fukui, 23-3 Shimoaizuki, Matsuoka, Fukui, Japan.
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MeSH Terms
Analysis of Variance
Brain / drug effects*
Choline O-Acetyltransferase / metabolism
Cholinesterase Inhibitors / therapeutic use*
Dose-Response Relationship, Drug
Indans / therapeutic use*
Infarction, Middle Cerebral Artery / complications
Piperidines / therapeutic use*
Rats, Sprague-Dawley
Time Factors
Urinary Bladder / drug effects
Urinary Bladder, Overactive / drug therapy*,  etiology
Reg. No./Substance:
0/Cholinesterase Inhibitors; 0/Indans; 0/Piperidines; 120011-70-3/donepezil; EC O-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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