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Acetylcholinesterase histochemistry of rectal suction biopsies in the diagnosis of Hirschsprung's disease.
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MedLine Citation:
PMID:  1299239     Owner:  NLM     Status:  MEDLINE    
Rectal suction biopsy with acetylcholinesterase (AChE) histochemistry has gained increased acceptance as the means of definitely diagnosing Hirschsprung's disease (HD) as well as of excluding this diagnosis when evaluating children with low intestinal obstruction or chronic constipation since the report of Meier-Ruge et al. in 1972. But this AChE histochemical study has not been reported yet in Korea. During the 14-month period from April, 1991 through June, 1992, 37 children, aged 3 days to 17 years had rectal suction biopsies for the diagnosis or exclusion of HD. In this study, AChE histochemistry (N = 37) was compared with hematoxylin & eosin (H&E) staining of same suction biopsy specimens (N = 35) for diagnostic accuracy. The histochemical criterion used for the diagnosis of Hirschsprung's disease was that of Chow et al. (1977), i.e., the presence of many coarse discrete cholinergic fibers in the muscularis mucosae and in the immediately subjacent submucosa regardless of an infiltration of cholinergic fibers in the lamina propria. Of 13 biopsies from the patients with Hirschsprung's disease (N = 13), there were 12 positive reactions, and one false negative reaction in a neonate with total colonic aganglionosis. All biopsies from 24 unaffected children demonstrated negative reactions with no false positive reaction. In comparison, of the 35 specimens examined by H&E staining, ganglion cells were present in the submucosal Meissner's plexus only in 15 of these 24 unaffected children. In conclusion, a 97% diagnostic accuracy was achieved with AChE histochemistry compared with a 74% accuracy with H&E staining (P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
W H Park; S O Choi; K Y Kwon; E S Chang
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Korean medical science     Volume:  7     ISSN:  1011-8934     ISO Abbreviation:  J. Korean Med. Sci.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-05-20     Completed Date:  1993-05-20     Revised Date:  2011-03-17    
Medline Journal Info:
Nlm Unique ID:  8703518     Medline TA:  J Korean Med Sci     Country:  KOREA    
Other Details:
Languages:  eng     Pagination:  353-9     Citation Subset:  IM    
Department of Surgery, Keimyung University Dongsan Medical Center, Taegu, Korea.
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MeSH Terms
Acetylcholinesterase / analysis*
Biopsy / methods
Child, Preschool
Hirschsprung Disease / enzymology,  pathology*
Histological Techniques
Infant, Newborn
Predictive Value of Tests
Rectum / enzymology,  pathology*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Korean Med Sci
Journal ID (pmc): jkms
ISSN: 1011-8934
ISSN: 1598-6357
Publisher: Korean Academy of Medical Sciences
Article Information
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Print publication date: Month: 12 Year: 1992
Volume: 7 Issue: 4
First Page: 353 Last Page: 359
ID: 3053840
PubMed Id: 1299239

Acetylcholinesterase histochemistry of rectal suction biopsies in the diagnosis of Hirschsprung's disease.
W. H. Park
S. O. Choi
K. Y. Kwon
E. S. Chang
Department of Surgery, Keimyung University Dongsan Medical Center, Taegu, Korea.

Article Categories:
  • Research Article

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