| Acetylcholine inhibits cell cycle progression in rat Schwann cells by activation of the M2 receptor subtype. | |
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MedLine Citation:
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PMID: 18634559 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Cultures of Schwann cells from neonatal rat sciatic nerves were treated with acetylcholine agonists and the effects on cell proliferation evaluated. (3)[H]-thymidine incorporation shows that acetylcholine (ACh) receptor agonists inhibit cell proliferation, and FACS analysis demonstrates cell-cycle arrest and accumulation of cells in the G1 phase. The use of arecaidine, a selective agonist of muscarinic M2 receptors reveals that this effect depends mainly on M2 receptor activation. The arecaidine dependent-block in G1 is reversible because removal of arecaidine from the culture medium induces progression to the S phase. The block of the G1-S transition is also characterized by modulation of the expression of several cell-cycle markers. Moreover, treatment with ACh receptor agonist causes both a decrease in the PCNA protein levels in Schwann cell nuclei and an increase in p27 and p53 proteins. Finally, immuno-electron microscopy demonstrates that M2 receptors are expressed by Schwann cells in vivo. These results indicate that ACh, by modulating Schwann cell proliferation through M2 receptor activation, might contribute to their progression to a more differentiated phenotype. |
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Authors:
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Simona Loreti; Ruggero Ricordy; M Egle De Stefano; Gabriella Augusti-Tocco; Ada Maria Tata |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuron glia biology Volume: 3 ISSN: 1741-0533 ISO Abbreviation: Neuron Glia Biol. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2008-07-18 Completed Date: 2009-12-14 Revised Date: 2013-01-22 |
Medline Journal Info:
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Nlm Unique ID: 101217278 Medline TA: Neuron Glia Biol Country: England |
Other Details:
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Languages: eng Pagination: 269-79 Citation Subset: - |
Affiliation:
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Department of Cell and Developmental Biology, University of Roma La Sapienza, Research Center of Neuroscience Daniel Bovet, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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