| Acetylcholine induces mesenchymal stem cell migration via Ca2+ /PKC/ERK1/2 signal pathway. | |
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MedLine Citation:
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PMID: 22441978 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acetylcholine (ACh) plays an important role in neural and non-neural function, but its role in mesenchymal stem cell (MSC) migration remains to be determined. In the present study, we have found that ACh induces MSC migration via muscarinic acetylcholine receptors (mAChRs). Among several mAChRs, MSCs express mAChR subtype 1 (m1AChR). ACh induces MSC migration via interaction with mAChR1. MEK1/2 inhibitor PD98059 blocks ERK1/2 phosphorylation while partially inhibiting the ACh-induced MSC migration. InsP3Rs inhibitor 2-APB that inhibits MAPK/ERK phosphorylation completely blocks ACh-mediated MSC migration. Interestingly, intracellular Ca(2+) ATPase-specific inhibitor thapsigargin also completely blocks ACh-induced MSC migration through the depletion of intracellular Ca(2+) storage. PKCα or PKCβ inhibitor or their siRNAs only partially inhibit ACh-induced MSC migration, but PKC-ζ siRNA completely inhibits ACh-induced MSC migration via blocking ERK1/2 phosphorylation. These results indicate that ACh induces MSC migration via Ca(2+), PKC, and ERK1/2 signal pathways. |
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Authors:
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Jun-Ming Tang; Jie Yuan; Qing Li; Jia-Ning Wang; Xia Kong; Fei Zheng; Lei Zhang; Long Chen; Lin-Yun Guo; Yong-Hang Huang; Jian-Ye Yang; Shi-You Chen |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 113 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-06-19 Completed Date: 2012-10-19 Revised Date: 2013-04-26 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 2704-13 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine, Hubei 442000, China. tangjm416@163.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology* Animals Blotting, Western Calcium / metabolism* Cell Movement / physiology Cell Proliferation / drug effects MAP Kinase Signaling System / drug effects* Male Mesenchymal Stromal Cells / cytology*, drug effects Protein Kinase C / metabolism* Rats Rats, Sprague-Dawley Receptors, Muscarinic / metabolism Signal Transduction / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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HL093429/HL/NHLBI NIH HHS; HL107526/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Muscarinic; 51-84-3/Acetylcholine; 7440-70-2/Calcium; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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