Document Detail

Acetylcholine exocytosis in PC12 cells deficient in SNAP-25.
MedLine Citation:
PMID:  9243624     Owner:  NLM     Status:  MEDLINE    
Stimulus-induced acetylcholine (ACh) exocytosis from presynaptic nerve terminals involves two important steps: fusion of ACh loaded vesicles at presynaptic release sites, followed by release into the synaptic cleft. We studied the role of the putative vesicle fusion protein SNAP-25 in this process. The nerve growth factor-differentiated PC12 cell line was used as an experimental model. A bee venom tetradecapeptide (INLKALAALAKKIL-NH2) phospholipase A2 (PLA2) activator, mastoparan, was used to induce ACh release. Treatment of PC12 cells with appropriate antisense oligonucleotides blocked SNAP-25 expression, as judged by Western blot protein analysis with a specific monoclonal antibody. Despite apparent elimination of SNAP-25, treatment of differentiated PC12 cells with mastoparan and high (80 mM) K+ induced ACh exocytosis. The results indicate that in PC12 cells, ACh exocytosis due to mastoparan plus K+ can occur in the absence of SNAP-25.
P Ray; C B Millard; J P Petrali; J D Berman; R Ray
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroreport     Volume:  8     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-10-07     Completed Date:  1997-10-07     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2271-4     Citation Subset:  IM    
Biology Department, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
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MeSH Terms
Acetylcholine / metabolism*
Blotting, Western
Exocytosis / drug effects,  physiology*
Membrane Proteins*
Nerve Tissue Proteins / metabolism*
Neurotoxins / pharmacology
PC12 Cells / metabolism*
Potassium Chloride / pharmacology
Synaptosomal-Associated Protein 25
Wasp Venoms / pharmacology
Reg. No./Substance:
0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/Neurotoxins; 0/Peptides; 0/Snap25 protein, rat; 0/Synaptosomal-Associated Protein 25; 0/Wasp Venoms; 51-84-3/Acetylcholine; 72093-21-1/mastoparan; 7447-40-7/Potassium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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