Document Detail

Acetylated sialic acid residues and blood group antigens localise within the epithelium in microvillous atrophy indicating internal accumulation of the glycocalyx.
MedLine Citation:
PMID:  15542511     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Microvillous atrophy, a disorder of intractable diarrhoea in infancy, is characterised by the intestinal epithelial cell abnormalities of abnormal accumulation of periodic acid-Schiff (PAS) positive secretory granules within the apical cytoplasm and the presence of microvillous inclusions. The identity of the PAS positive material is not known, and the aim of this paper was to further investigate its composition.
METHODS: Formaldehyde fixed sections were stained with alcian blue/PAS to identify the acidic or neutral nature of the material, phenylhydrazine blocking was employed to stain specifically for sialic acid, and saponification determined the presence of sialic acid acetylation. The specificity of sialic acid staining was tested by digestion with mild sulphuric acid. Expression of blood group related antigens was tested immunochemically.
RESULTS: Alcian blue/PAS staining identified a closely apposed layer of acidic material on the otherwise neutral (PAS positive) brush border in controls. In microvillous atrophy, a triple layer was seen with an outer acidic layer, an unstained brush border region, and accumulation within the epithelium of a neutral glycosubstance that contained acetylated sialic acid. Blood group antigens were detected on the brush border, in mucus, and within goblet cells in controls. In microvillous atrophy they were additionally expressed within the apical cytoplasm of epithelial cells mirroring the PAS abnormality. Immuno electron microscopy localised expression to secretory granules.
CONCLUSIONS: A neutral, blood group antigen positive, glycosubstance that contains acetylated sialic acid accumulates in the epithelium in microvillous atrophy. Previous studies have demonstrated that the direct and indirect constitutive pathways are intact in this disorder and it is speculated that the abnormal staining pattern reflects accumulation of glycocalyx related material.
A D Phillips; A Brown; S Hicks; S Schüller; S H Murch; J A Walker-Smith; D M Swallow
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gut     Volume:  53     ISSN:  0017-5749     ISO Abbreviation:  Gut     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-15     Completed Date:  2004-12-14     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  2985108R     Medline TA:  Gut     Country:  England    
Other Details:
Languages:  eng     Pagination:  1764-71     Citation Subset:  AIM; IM    
Centre for Paediatric Gastroenterology, Department of Paediatrics and Child Health, Royal Free Hospital, Pond St, London NW3 2QG, UK.
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MeSH Terms
ABO Blood-Group System / metabolism
Atrophy / congenital,  metabolism
Blood Group Antigens / metabolism*
Child, Preschool
Diarrhea / metabolism,  pathology
Glycocalyx / metabolism*
Intestinal Mucosa / metabolism,  pathology*
Intestine, Small / metabolism,  pathology
Lewis Blood-Group System / metabolism
Microscopy, Electron
Microvilli / pathology
Sialic Acids / metabolism*
Reg. No./Substance:
0/ABO Blood-Group System; 0/Blood Group Antigens; 0/Lewis Blood-Group System; 0/Sialic Acids

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