Document Detail

Acetyl-CoA carboxylase and fatty acid synthase activity and immunodetectable protein in adipose tissues of ruminants: effect of temperature and feeding level.
MedLine Citation:
PMID:  10985414     Owner:  NLM     Status:  MEDLINE    
To gain insights into the regulation of fat synthesis, we have investigated the effect of cold environmental exposure and feed restriction of sheep on activity and immunodetectable protein content of acetyl-CoA carboxylase (ACC) and fatty acid synthase in adipose tissue. Subcutaneous and mesenteric adipose tissues were collected at slaughter from sheep exposed to either cold (0+/-2 degrees C) or warm (23+/-2 degrees C) environment, and given either ad libitum or restricted access to feed for three 5-wk periods. Acetyl-CoA carboxylase was isolated from frozen adipose tissue samples and activity determined as the rate of incorporation of H14CO3- into acid stable malonyl-CoA. Cold exposure and feed restriction reduced (P < .05) ACC activity in the two adipose tissue depots. Western blot analysis with peroxidase-conjugated streptavidin showed that both adipose tissue depots express a single isoform of ACC. In s.c. adipose tissue, cold exposure increased (P < .05) ACC protein abundance, which is opposite to the change in activity. However, feed restriction reduced immunodetectable ACC protein. There was no significant effect of environment or feeding level on ACC protein abundance in mesenteric tissue. Fatty acid synthase activity determined in ammonium sulfate extract by measuring the malonyl-CoA- and acetyl-CoA-dependent oxidation of NADPH was decreased (P < .05) by feed restriction in both s.c. and mesenteric tissues. Cold exposure reduced fatty acid synthase activity in s.c. but not in mesenteric tissue. There was no effect of environment on fatty acid synthase protein abundance in either adipose tissue depot. However, feed restriction significantly reduced fatty acid synthase protein abundance in the two depots. The data suggest that feed restriction and exposure of ruminants to cold environmental conditions may significantly down-regulate the activity of key lipogenic enzymes.
J A Moibi; E D Ekpe; R J Christopherson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of animal science     Volume:  78     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-12-19     Completed Date:  2000-12-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2383-92     Citation Subset:  IM    
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
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MeSH Terms
Acetyl-CoA Carboxylase / metabolism*
Adipose Tissue / enzymology*
Animal Feed*
Blotting, Western / veterinary
Cold Temperature*
Electrophoresis, Polyacrylamide Gel / veterinary
Energy Intake
Fatty Acid Synthetase Complex / metabolism*
Housing, Animal
Sheep / metabolism*
Reg. No./Substance:
EC 6.-/Fatty Acid Synthetase Complex; EC Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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