| Acetic acid recovery of gynecologic liquid-based samples of apparent low squamous cellularity. | |
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MedLine Citation:
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PMID: 16610679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To characterize cervicovaginal cytology samples with < 5,000 squamous cells on the initial ThinPrep slide (Cytyc Corp., Boxborough, Massachusetts, U.S.A) and to attempt sample recovery using acetic acid. STUDY DESIGN: Cervicovaginal cytology samples with <5,000 squamous cells on the original ThinPrep slide and residuum were reprocessed by adding 3 mL of 3:1 CytoLyt (Cytyc)/glacial acetic acid with production of a second slide. Both slides were reviewed for squamous cell quantitation and the presence of background material and abnormal cells. RESULTS: From a total of 1,833 cases, 147 (8.0%) were identified for reprocessing; 71 (48.3%) were grossly bloody and 58 (39.4%) grossly cloudy. Reprocessing resulted in a second slide with > 5,000 squamous cells in 116 (78.9%) cases and was most effective on cloudy samples (89.7% recovery) and bloody samples (71.8% recovery). Abnormal cells were identified in 13 (8.9%) reprocessed samples. In all but 2 cases the abnormal cells were present on the initial slide and demonstrated the same degree of abnormality as the reprocessed slide but were fewer in number. CONCLUSION: Acetic acid recovery increases squamous cell recovery when initially inadequate, reducing the number of unsatisfactory cases and in rare cases identifying a cytologically significant lesion not apparent on the original slide. |
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Authors:
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Pamela Dalton; Scott MacDonald; Scott Boerner |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta cytologica Volume: 50 ISSN: 0001-5547 ISO Abbreviation: Acta Cytol. Publication Date: 2006 Mar-Apr |
Date Detail:
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Created Date: 2006-04-13 Completed Date: 2006-05-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370307 Medline TA: Acta Cytol Country: United States |
Other Details:
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Languages: eng Pagination: 136-40 Citation Subset: IM |
Affiliation:
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Department of Pathology, Toronto Medical Laboratories, University Health Network, Toronto, Ontario, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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chemistry* Carcinoma, Squamous Cell / diagnosis*, pathology Cervical Intraepithelial Neoplasia / diagnosis*, pathology Female Humans Sensitivity and Specificity Vaginal Smears / methods*, standards |
| Chemical | |
Reg. No./Substance:
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64-19-7/Acetic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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