Document Detail

Acetic acid recovery of gynecologic liquid-based samples of apparent low squamous cellularity.
MedLine Citation:
PMID:  16610679     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To characterize cervicovaginal cytology samples with < 5,000 squamous cells on the initial ThinPrep slide (Cytyc Corp., Boxborough, Massachusetts, U.S.A) and to attempt sample recovery using acetic acid. STUDY DESIGN: Cervicovaginal cytology samples with <5,000 squamous cells on the original ThinPrep slide and residuum were reprocessed by adding 3 mL of 3:1 CytoLyt (Cytyc)/glacial acetic acid with production of a second slide. Both slides were reviewed for squamous cell quantitation and the presence of background material and abnormal cells. RESULTS: From a total of 1,833 cases, 147 (8.0%) were identified for reprocessing; 71 (48.3%) were grossly bloody and 58 (39.4%) grossly cloudy. Reprocessing resulted in a second slide with > 5,000 squamous cells in 116 (78.9%) cases and was most effective on cloudy samples (89.7% recovery) and bloody samples (71.8% recovery). Abnormal cells were identified in 13 (8.9%) reprocessed samples. In all but 2 cases the abnormal cells were present on the initial slide and demonstrated the same degree of abnormality as the reprocessed slide but were fewer in number. CONCLUSION: Acetic acid recovery increases squamous cell recovery when initially inadequate, reducing the number of unsatisfactory cases and in rare cases identifying a cytologically significant lesion not apparent on the original slide.
Pamela Dalton; Scott MacDonald; Scott Boerner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta cytologica     Volume:  50     ISSN:  0001-5547     ISO Abbreviation:  Acta Cytol.     Publication Date:    2006 Mar-Apr
Date Detail:
Created Date:  2006-04-13     Completed Date:  2006-05-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370307     Medline TA:  Acta Cytol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  136-40     Citation Subset:  IM    
Department of Pathology, Toronto Medical Laboratories, University Health Network, Toronto, Ontario, Canada.
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MeSH Terms
Acetic Acid / chemistry*
Carcinoma, Squamous Cell / diagnosis*,  pathology
Cervical Intraepithelial Neoplasia / diagnosis*,  pathology
Sensitivity and Specificity
Vaginal Smears / methods*,  standards
Reg. No./Substance:
64-19-7/Acetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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