Document Detail

The acellular myocardial flap: a novel extracellular matrix scaffold enriched with patent microvascular networks and biocompatible cell niches.
MedLine Citation:
PMID:  23151037     Owner:  NLM     Status:  MEDLINE    
There is a great need for acellular, fully vascularized, and biocompatible myocardial scaffolds that provide agreeable biological, nutritional, and biomechanical niches for reseeded cells for in vitro and in vivo applications. We generated myocardial flap scaffolds comprising porcine left-anterior ventricular myocardium and its associated coronary arteries and veins and investigated the combinatorial effects of sodium dodecyl sulfate (SDS) and sodium hydroxide (NaOH) perfusion on both the myocardial extracellular matrix (ECM) and the vascular ECM. Results showed that all scaffolds displayed a fully intact and patent vasculature, with arterial burst pressures indistinguishable from native coronary arteries and perfusion to the level of capillaries. Scaffolds were free of cellular proteins and retained collagen and elastin ECM components, exhibited excellent mechanical properties, and were cytocompatible toward relevant seeded cells. SDS perfusion preserved collagen IV, laminin, and fibronectin well, but only reduced DNA content by 33%; however, this was further improved by post-SDS nuclease treatments. By comparison, NaOH was very effective in removing cells and eliminated more than 95% of tissue DNA, but also significantly reduced levels of laminin and fibronectin. Such constructs can be readily trimmed to match the size of the infarct and might be able to functionally integrate within host myocardium and be nourished by direct anastomotic connection with the host's own vasculature; they might also be useful as physiologically accurate models for in vitro studies of cardiac physiology and pathology.
Jason B Schulte; Agneta Simionescu; Dan T Simionescu
Related Documents :
17376747 - Acute heart failure and near-syncope associated with giant left atrial ball thrombus oc...
17670077 - Coexisting chordal papillary fibroelastoma and leaflet lambl's tumour of the mitral valve.
22322367 - Three potential mechanisms for failure of high intensity focused ultrasound ablation in...
8678707 - Thrombosis in ischemic heart disease.
7959557 - Major hepatic resection in patients with a prosthetic heart valve receiving anticoagula...
20185107 - Biventricular stimulation improves right and left ventricular function after tetralogy ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-01-16
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  19     ISSN:  1937-3392     ISO Abbreviation:  Tissue Eng Part C Methods     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-05-23     Completed Date:  2014-04-16     Revised Date:  2014-07-01    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  518-30     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Basement Membrane / drug effects,  metabolism
Biocompatible Materials / pharmacology*
Biomechanical Phenomena / drug effects
Collagen / metabolism
Coronary Vessels / cytology
Elastin / metabolism
Extracellular Matrix / drug effects,  metabolism*
Fibronectins / metabolism
Materials Testing
Microvessels / cytology*,  drug effects
Myocardium / cytology*
Sus scrofa
Tissue Scaffolds / chemistry*
Vascular Patency / drug effects
Grant Support
Reg. No./Substance:
0/Biocompatible Materials; 0/Fibronectins; 9007-34-5/Collagen; 9007-58-3/Elastin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Nitric oxide and superoxide mediate diesel particle effects in cytokine-treated mice and murine lung...
Next Document:  Deciphering the chemical bonding in anionic thallium clusters.