| Accurate, reproducible measurement of acetone concentration in breath using selected ion flow tube-mass spectrometry. | |
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MedLine Citation:
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PMID: 21383484 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Using selected ion flow tube-mass spectrometry (SIFT-MS) for on-line analysis, we aimed to define the optimal single-exhalation breathing manoeuvre from which a measure of expired acetone concentration could be obtained. Using known acetone concentrations in vitro, we determined the instrument's accuracy, inter-measurement variability and dynamic response time. Further, we determined the effects of expiratory flow and volume on acetone concentration in the breath of 12 volunteers and calculated intra-individual coefficients of variation (CVs). At acetone concentrations of 600-3000 ppb on 30 days over 2 months there was an instrument measurement bias of 8% that did not change over time, inter-day and intra-day CVs were 5.6% and 0.0%, respectively, and the 10-90% response time was 500 ± 50 ms (mean ± SE). Acetone concentrations at exhalation flows of 193 ± 18 (mean ± SD) and 313 ± 32 ml s(-1) were 619 ± 1.83 (geometric mean ± logSD) and 618 ± 1.82 ppb in the fraction 70-85% by volume of exhaled vital capacity (V(70-85%)) and 636 ± 1.82 (geometric mean ± logSD) and 631 ± 1.83 ppb in V(85-100%). A difference was observed between acetone concentrations in the V(70-85%) and V(85-100%) fractions (p < 0.01), but flow had no effect. Median intra-individual CVs were 1.6-2.6%. On-line SIFT-MS measurement of acetone concentration in a single exhalation requires control of exhaled volume but not flow, and yields low intra-individual CVs and is potentially useful in approximating blood glucose and monitoring metabolic stress. |
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Authors:
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Jack F Dummer; Malina K Storer; Wan-Ping Hu; Maureen P Swanney; Gordon J Milne; Christopher M Frampton; Jenny M Scotter; G Kim Prisk; Michael J Epton |
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Publication Detail:
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Type: Journal Article Date: 2010-11-15 |
Journal Detail:
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Title: Journal of breath research Volume: 4 ISSN: 1752-7163 ISO Abbreviation: J Breath Res Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2011-03-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101463871 Medline TA: J Breath Res Country: England |
Other Details:
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Languages: eng Pagination: 046001 Citation Subset: IM |
Affiliation:
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Respiratory Services, Christchurch Hospital, Christchurch, New Zealand. Department of Medicine, University of Otago, Christchurch, New Zealand. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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