Document Detail

Accurate photodensitometric measurement of the lecithin: sphingomyelin ration after elimination of the acidic phospholipids from extracts of amniotic fluid specimens.
MedLine Citation:
PMID:  589800     Owner:  NLM     Status:  MEDLINE    
Treatment of human amniotic fluid lipids dissolved in a chloroform/methanol (9:1, v/v) mixture by batchwise addition of diethylaminoethyl cellulose in a dry state proved to be an easy and rapid procedure for the removal of the acidic phospholipids which may interfere in the photodensitometric evaluation of the lecithin: spingomyelin ratio on a thin-layer chromatogram. This method was used for the measurement of the lecithin: sphingomyelin ratio in a series of normal and abnormal pregnancies. Ratios higher than expected from gestational age were observed in stressed pregnancies. A significant elevation of the ratio was also observed under treatment by dexamethasone.
L Gosselin; J M Foidart
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  81     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1977 Dec 
Date Detail:
Created Date:  1978-02-18     Completed Date:  1978-02-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  209-14     Citation Subset:  IM    
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MeSH Terms
Amniotic Fluid / analysis*
Chromatography, Thin Layer
Densitometry / methods
Gestational Age
Phosphatidylcholines / analysis*
Phospholipids / isolation & purification*
Sphingomyelins / analysis*
Reg. No./Substance:
0/Phosphatidylcholines; 0/Phospholipids; 0/Sphingomyelins

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