Document Detail


Accurate and highly reproducible picoliter injection system for capillary electrophoresis.
MedLine Citation:
PMID:  23153061     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A novel, highly-accurate sample injection system for capillary electrophoresis (CE) was developed based on ink-jet microchip capable of reproducing exact introduction volumes at the picoliter level. The difficulty in analyte discrimination using electrokinetic injection was also overcome using this injection method. The injection system consisted of an XY stage, an ink-jet droplet ejection microchip, and a reservoir with a plug-in septum. To evaluate the precision of the system, a mixture of NBD-labeled amino acids consisting of Gly, L-Phe, L-Asp, and L-Ser were separated, and the performance was compared with that of traditional hydrodynamic and electrokinetic injection methods. The results demonstrated the introduced volume was highly relied on the number of droplets with low relative standard derivation (RSD) and good linear correction coefficient in the proposed injection method. In addition, a urine sample was analyzed via CE coupled with the ink-jet injection system for the detection of the amino acid taurine. The concentration of urinary taurine was determined to be 2.42 ± 0.08 μM (confidence level, 95%; RSD, 1.05%; n = 4) with a recovery of 98.92-109.54% (n = 3). These results demonstrate the ink-jet injection system we developed has the potential to revolutionize capillary electrophoretic separation in practical and commercial applications that require an automated accurate injection system.
Authors:
Hulie Zeng; Ying Weng; Saori Ikeda; Yuri Nakagawa; Hizuru Nakajima; Katsumi Uchiyama
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-16
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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