Document Detail


Accurate analysis of urea in milk and milk powder by isotope dilution gas chromatography-mass spectrometry.
MedLine Citation:
PMID:  20434964     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A high order method for measuring urea concentrations in milk and milk powder was developed. The method can be applied to certify the concentration of urea in some new milk and milk powder CRMs. This high accurate method for analysis of milk is valuable given the inherent challenges associated with the complexity of the sample matrix. A measurement procedure based on gas chromatography/isotope dilution mass spectrometry (GC/IDMS) was developed. Samples were pre-treated with acetonitrile to remove proteins and the method was applied to determine urea concentrations in milk and milk powder. Excellent precision was obtained, with within- and between-set coefficients of variation of 0.15-0.46 and 0.18-0.65%, respectively. The measurement uncertainty is evaluated. The method can trace to mass.
Authors:
Xinhua Dai; Xiang Fang; Fuhai Su; Mengrui Yang; Hongmei Li; Jian Zhou; Ruifeng Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-14
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  878     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-17     Completed Date:  2010-08-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1634-8     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
National Institute of Metrology, No. 18 Beisanhuan Donglu, Chaoyang Dist, Beijing 100013, PR China. xhdai_75@iccas.ac.cn
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MeSH Terms
Descriptor/Qualifier:
Acetonitriles
Animals
Gas Chromatography-Mass Spectrometry / methods*
Isotope Labeling / methods*
Linear Models
Methanol
Milk / chemistry*
Reproducibility of Results
Sensitivity and Specificity
Urea / analysis*
Chemical
Reg. No./Substance:
0/Acetonitriles; 57-13-6/Urea; 67-56-1/Methanol; 75-05-8/acetonitrile

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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