| Accuracy of image registration between MRI and light microscopy in the ex vivo brain. | |
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MedLine Citation:
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PMID: 21546191 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A multistep procedure was developed to register magnetic resonance imaging (MRI) and histological data from the same sample in the light microscopy image space, with the ultimate goal of allowing quantitative comparisons of the two datasets. The fixed brain of an owl monkey was used to develop and test the procedure. In addition to the MRI and histological data, photographic images of the brain tissue block acquired during sectioning were assembled into a blockface volume to provide an intermediate step for the overall registration process. The MR volume was first registered to the blockface volume using a combination of linear and nonlinear registration, and two dimensional (2D) blockface sections were registered to corresponding myelin-stained sections using a combination of linear and nonlinear registration. Before this 2D registration, two major types of tissue distortions were corrected: tissue tearing and independent movement of different parts of the brain, both introduced during histological processing of the sections. The correction procedure utilized a 2D method to close tissue tears and a multiple iterative closest point (ICP) algorithm to reposition separate pieces of tissue in the image. The accuracy of the overall MR to micrograph registration procedure was assessed by measuring the distance between registered landmarks chosen in the MR image space and the corresponding landmarks chosen in the micrograph space. The average error distance of the MR data registered to micrograph data was 0.324±0.277 mm, only 8% larger than the width of the MRI voxel (0.3 mm). |
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Authors:
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Ann S Choe; Yurui Gao; Xia Li; Keegan B Compton; Iwona Stepniewska; Adam W Anderson |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-05-05 |
Journal Detail:
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Title: Magnetic resonance imaging Volume: 29 ISSN: 1873-5894 ISO Abbreviation: Magn Reson Imaging Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-23 Completed Date: 2011-09-15 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 8214883 Medline TA: Magn Reson Imaging Country: Netherlands |
Other Details:
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Languages: eng Pagination: 683-92 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Vanderbilt University Institute of Imaging Science, Nashville, TN, USA. annschoe@gmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Aotidae Brain / pathology* Brain Mapping / methods Image Processing, Computer-Assisted / methods* Imaging, Three-Dimensional / methods Magnetic Resonance Imaging / methods* Microscopy / methods* Myelin Sheath / chemistry Reproducibility of Results |
| Grant Support | |
ID/Acronym/Agency:
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1R01EB002777/EB/NIBIB NIH HHS; 1R01NS58639/NS/NINDS NIH HHS; 1S10RR17799/RR/NCRR NIH HHS; 5R01DA028303-02/DA/NIDA NIH HHS; R01 NS058639-01A1/NS/NINDS NIH HHS; S10 RR017799-01/RR/NCRR NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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