Document Detail

Accuracy of BRT and Delvotest microbial inhibition tests as affected by composition of ewe's milk.
MedLine Citation:
PMID:  12636303     Owner:  NLM     Status:  MEDLINE    
The presence of drug residues in ewe's milk samples can be determined by microbial assays. The main limitation of these tests is the large number of false-positive results associated with them. False-positive results can be explained by the interaction of certain substances naturally existing in ewe's milk with the growth of the microorganism used in the test. In this study, milk chemical composition (fat, protein, lactose, total solids), somatic cell counts (SCCs), free fatty acid concentrations, and lactoperoxidase system components were determined in order to investigate their influence on the rate of false-positive results for the BRT and Delvotest microbiological inhibitor tests. Milk samples were obtained after morning milking of Manchega ewes at 15, 30, 45, 60, 75, 90, 105, 120, and 135 days after parturition. The animals did not receive any kind of treatment or medicated feed throughout the experiment. The false-positive rates for BRT and Delvotest were 3.75 and 2.4%, respectively. When the logistic regression model was applied, the percentages of total solids for positive samples were significantly different from those for negative samples (16.90 versus 18.42% for BRT, 16.05 versus 18.45% for Delvotest), while the SCC logarithmic transformation was significantly higher for the positive samples than for the negative samples (5.38 versus 5.11 log units for BRT, 5.32 versus 5.11 log units for Delvotest). Moreover, Delvotest-positive samples exhibited thiocyanate concentrations higher than those of Delvotest-negative samples (8.18 mg/liter versus 6.85 mg/liter). Further analyses are needed to confirm the possible presence of antimicrobial residues in this particular type of milk sample.
Rafael Althaus; Antonio Torres; Cristofol Peris; M Carmen Beltran; Nemesio Fernandez; M Pilar Molina
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  66     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-14     Completed Date:  2003-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  473-8     Citation Subset:  IM    
Departamento de Ciencia Animal, Universidad Politécnica de Valencia, Camino de Vera, 14, Apartado 22012, 46071 Valencia, Spain.
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MeSH Terms
Anti-Bacterial Agents / analysis
Cell Count
Drug Residues / analysis*
False Positive Reactions
Food Contamination / analysis*
Microbial Sensitivity Tests / methods*
Milk / chemistry*,  cytology,  microbiology
Sensitivity and Specificity
Reg. No./Substance:
0/Anti-Bacterial Agents

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