Document Detail


Accumulation of theanderose in association with development of freezing tolerance in the moss Physcomitrella patens.
MedLine Citation:
PMID:  16527318     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mosses are known to have the ability to develop high degrees of resistance to desiccation and freezing stress at cellular levels. However, underlying cellular mechanisms leading to the development of stress resistance in mosses are not understood. We previously showed that freezing tolerance in protonema cells of the moss Physcomitrella patens was rapidly increased by exogenous application of the stress hormone abscisic acid (ABA) [Minami, A., Nagao, M., Arakawa, K., Fujikawa, S., Takezawa, D., 2003a. Abscisic acid-induced freezing tolerance in the moss Physcomitrella patens is accompanied by increased expression of stress-related genes. J. Plant Physiol. 160, 475-483]. Herein it is shown that protonema cells with acquired freezing tolerance specifically accumulate low-molecular-weight soluble sugars. Analysis of the most abundant trisaccharide revealed that the cells accumulated theanderose (G6-alpha-glucosyl sucrose) in close association with enhancement of freezing tolerance by ABA treatment. The accumulation of theanderose was inhibited by cycloheximide, an inhibitor of nuclear-encoded protein synthesis, coinciding with a remarkable decrease in freezing tolerance. Furthermore, theanderose accumulation was promoted by cold acclimation and treatment with hyperosmotic solutes, both of which had been shown to enhance cellular freezing tolerance. These results reveal a novel role for theanderose, whose biological function has been obscure, in high freezing tolerance in moss cells.
Authors:
Manabu Nagao; Kazuyuki Oku; Anzu Minami; Kaoru Mizuno; Minoru Sakurai; Keita Arakawa; Seizo Fujikawa; Daisuke Takezawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-09
Journal Detail:
Title:  Phytochemistry     Volume:  67     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-27     Completed Date:  2006-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  702-9     Citation Subset:  IM    
Affiliation:
Cryobiosystem Research Center, Iwate University, Morioka 020-8550, Japan.
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / pharmacology
Bryopsida / cytology,  physiology*
Chromatography, High Pressure Liquid
Cycloheximide / pharmacology
Freezing
Gene Expression Regulation, Plant*
Magnetic Resonance Spectroscopy
Mannitol / pharmacology
Molecular Weight
Osmolar Concentration
Protein Synthesis Inhibitors / pharmacology
Sodium Chloride / pharmacology
Trisaccharides / metabolism*,  physiology
Chemical
Reg. No./Substance:
0/Protein Synthesis Inhibitors; 0/Trisaccharides; 0/theanderose; 21293-29-8/Abscisic Acid; 66-81-9/Cycloheximide; 69-65-8/Mannitol; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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