Document Detail


Accumulation and storage of Zn2+ by Candida utilis.
MedLine Citation:
PMID:  6625     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Starved cells of Candida utilis accumulated Zn2+ by two different processes. The first was a rapid, energy- and temperature-independent system that probably represented binding to the cell surface. The cells also possessed an energy-, pH-, and temperature-dependent system that was capable of accumulating much greater quantities of the cation than the binding process. The energy-dependent system was inhibited by KCN, Na2HAsO4, m-chlorophenyl carbonylcyanide hydrazone, N-ethylmaleimide, EDTA and diethylenetriaminepenta-acetic acid. The system was specific inasmuch as Ca2+, Cr3+, Mn2+, Co2+ or Cu2+ did not compete with, inhibit, or enhance the process, Zn2+ uptake was inhibited by Cd2+. The system exhibited saturation kinetics with a half-saturation value of 1.3 muM and a maximum rate of 0.21 (nmol Zn2+) min(-1) (mg dry wt(-1)) at 30 degrees C. Zn2+ uptake required intact membranes since only the binding process was observed in the presence of nystatin, toluene, or sodium dodecyl sulphate. Cells did not exchange recently accumulated toluene, or sodium dodecyl sulphate. Cells did not exchange recently accumulated 65Zn following the addition of a large excess of non-radioactive Zn2+. Similarly, cells pre-loaded with 65Zn did not lose the cation during starvation, and efflux did not occur when glucose and exogenous Zn2+ were supplied after the starvation period. Efflux was only observed after the addition of toluene or nystatin, or when cells were heated to 100 degrees C. Cells fed a large quantity of Zn2+ contained a protein fraction resembling animal cell metallothionein. In batch culture, cells of C. utilis accumulated Zn2+ only during the lag phase and the latter half of the exponential-growth phase.
Authors:
M L Failla; C D Benedict; E D Weinberg
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of general microbiology     Volume:  94     ISSN:  0022-1287     ISO Abbreviation:  J. Gen. Microbiol.     Publication Date:  1976 May 
Date Detail:
Created Date:  1976-08-23     Completed Date:  1976-08-23     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  0375371     Medline TA:  J Gen Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  23-36     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Arsenates / pharmacology
Candida / metabolism*
Cell Membrane / metabolism
Cyanides / pharmacology
Edetic Acid / pharmacology
Electron Transport
Ethylmaleimide / pharmacology
Formaldehyde / pharmacology
Fungal Proteins / biosynthesis
Glucose / metabolism
Hydrazones
Hydrogen-Ion Concentration
Kinetics
Nystatin / pharmacology
Pentetic Acid / pharmacology
Temperature
Toluene / pharmacology
Uncoupling Agents / pharmacology
Zinc / metabolism*
Chemical
Reg. No./Substance:
0/Arsenates; 0/Cyanides; 0/Fungal Proteins; 0/Hydrazones; 0/Uncoupling Agents; 108-88-3/Toluene; 128-53-0/Ethylmaleimide; 1400-61-9/Nystatin; 50-00-0/Formaldehyde; 50-99-7/Glucose; 60-00-4/Edetic Acid; 67-43-6/Pentetic Acid; 7440-66-6/Zinc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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